| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
PDGFRA |
| Specificity: |
This antibody reacts specifically with PDGFRA |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human PDGF-R-A |
| Gene ID: |
5156 |
| Swiss Prot: |
P16234 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1, Binding of its cognate ligands - homodimeric PDGFA, Depending on the context, Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, PLCG1, Phosphorylates PIK3R1, Phosphorylates PIK3R1, Plays a role in cell migration and chemotaxis in wound healing, Plays a role in platelet activation, Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells, Promotes activation of STAT family members STAT1, Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, Required for normal development of the mucosa lining the gastrointestinal tract, Required for normal skeleton development and cephalic closure during embryonic development, STAT3 and STAT5A and/or STAT5B, and PTPN11, and by rapid internalization of the activated receptor, and for recruitment of mesenchymal cells and normal development of intestinal villi, and in thrombin-induced platelet aggregation, and thereby mediates activation of the AKT1 signaling pathway, cell proliferation, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades, homodimeric PDGFB, mobilization of cytosolic Ca(2+) and the activation of protein kinase C, promotes or inhibits cell proliferation and cell migration, secretion of agonists from platelet granules, survival and chemotaxis, the regulatory subunit of phosphatidylinositol 3-kinase, the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB, 4, 5-trisphosphate, Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
CD140a PDGFR2 GAS9 |
| Also known as: |
PDGFRA Polyclonal Antibody |
| Other name: |
Anti-PDGFRA Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
PDGFRA |
| Short name: |
Anti-PDGFRA (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, a polypeptide (polyclonal), antibody to-platelet-derived growth factor receptor |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
CD140A and PDGFR-2 and PDGFR2 and RHEPDGFRA, PDGFRA and IDBG-18854 and ENSG00000134853 and 5156, PDGFRA and IDBG-642308 and ENSBTAG00000007173 and 282301, Pdgfra and IDBG-171929 and ENSMUSG00000029231 and 18595, alpha polypeptide, nuclei, this GO :0001553 and luteinization and biological process this GO :0001701 and in utero embryonic development and biological process this GO :0001775 and cell activation and biological process this GO :0002244 and hematopoietic progenitor cell differentiation and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0005018 and platelet-derived growth factor alpha-receptor activity and molecular function this GO :0005021 and vascular endothelial growth factor-activated receptor activity and molecular function this GO :0005161 and platelet-derived growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0005902 and microvillus and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007204 and positive regulation of cytosolic calcium ion concentration and biological process this GO :0008210 and estrogen metabolic process and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0008585 and female this GO nad development and biological process this GO :0009653 and anatomical structure morphogenesis and biological process this GO :0009887 and organ morphogenesis and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010544 and negative regulation of platelet activation and biological process this GO :0010863 and positive regulation of phospholipase C activity and biological process this GO :0014068 and positive regulation of phosphatidylinositol 3-kinase signaling and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0016477 and cell migration and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004714 : transmembrane receptor protein tyrosine kinase activity and also this GO :0005018 : platelet-derived growth factor alpha-receptor activity and also this GO :0005021 : vascular endothelial growth factor-activated receptor activity and also this GO :0005161 : platelet-derived growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004714 : transmembrane receptor protein tyrosine kinase activity, this GO :0005018 : platelet-derived growth factor alpha-receptor activity, this GO :0005021 : vascular endothelial growth factor-activated receptor activity, this GO :0005161 : platelet-derived growth factor receptor binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, this GO :0038085 : vascular endothelial growth factor binding, this GO :0042803 : protein homodimerization activity, this GO :0048407 : platelet-derived growth factor binding, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0038085 : vascular endothelial growth factor binding and also this GO :0042803 : protein homodimerization activity and also this GO :0048407 : platelet-derived growth factor binding, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0023019 and signal transduction involved in regulation of gene expression and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030324 and lung development and biological process this GO :0030325 and adrenal gland development and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030539 and male genitalia development and biological process this GO :0031226 and intrinsic component of plasma membrane and cellular component this GO :0033327 and Leydig cell differentiation and biological process this GO :0035790 and platelet-derived growth factor receptor-alpha signaling pathway and biological process this GO :0038085 and vascular endothelial growth factor binding and molecular function this GO :0038091 and positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042060 and wound healing and biological process this GO :0042475 and odontogenesis of dentin-containing tooth and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0043552 and positive regulation of phosphatidylinositol 3-kinase activity and biological process this GO :0045087 and innate immune response and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0048008 and platelet-derived growth factor receptor signaling pathway and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048015 and phosphatidylinositol-mediated signaling and biological process this GO :0048146 and positive regulation of fibroblast proliferation and biological process this GO :0048407 and platelet-derived growth factor binding and molecular function this GO :0048557 and embryonic digestive tract morphogenesis and biological process this GO :0048701 and embryonic cranial skeleton morphogenesis and biological process this GO :0048704 and embryonic skeletal system morphogenesis and biological process this GO :0048705 and skeletal system morphogenesis and biological process this GO :0050920 and regulation of chemotaxis and biological process this GO :0055003 and cardiac myofibril assembly and biological process this GO :0060021 and palate development and biological process this GO :0060325 and face morphogenesis and biological process this GO :0060326 and cell chemotaxis and biological process this GO :0061298 and retina vasculature development in camera-type eye and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070527 and platelet aggregation and biological process this GO :0071230 and cellular response to amino acid stimulus and biological process this GO :0072277 and metanephric glomerular capillary formation and biological process this GO :2000249 and regulation of actin cytoskeleton reorganization and biological process this GO :2000739 and regulation of mesenchymal stem cell differentiation and biological process, platelet-derived growth factor receptor |
| Identity: |
8803 |
| Gene: |
PDGFRA |
More about : PDGFRA |
| Long gene name: |
platelet derived growth factor receptor alpha |
| Synonyms gene name: |
alpha polypeptide , platelet-derived growth factor receptor |
| Locus: |
4q12 |
| Discovery year: |
1989-05-19 |
| GenBank acession: |
D50001 |
| Entrez gene record: |
5156 |
| Pubmed identfication: |
8643452 |
| RefSeq identity: |
NM_006206 |
| Classification: |
I-set domain containing CD molecules Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000128699 |
| Locus Specific Databases: |
LRG_309 |