| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CENPA |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
1-0, 5ug/ml, Western blot: 0 |
| Notes: |
Optimal dilution of the CENPA antibody should be determined by the researcher |
| Intented use: |
This CENPA antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P49450 |
| Purity: |
Antigen affinity |
| Description: |
Alternative splicing results in multiple transcript variants encoding distinct isoforms, And CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle, CENP-A is one of the epigenetic changes that is believed to distinguish centromeric DNA from other DNA, CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere, In S, In higher eukaryotes, Once the CENP-A has been added, The RNAi is cut to form siRNA, This cohesin is believed to be essential in replacing the centromere H3 with CENP-A, This helps recruit other proteins, and by an RNAi mechanism, and the surrounding heterochromatin/RNAi mechanism is no longer necessary, de novo recruitment of the CENP-A to the centromere is believed to be controlled by centromeric heterochromatin surrounding the centromere, independent of the underlying DNA sequence, the centromere becomes self-propagating, the recruitment of CENP-A nucleosomes to existing centromeres is an epigenetic process, this complexes with the protein Chp1, ultimately resulting in a protein complex that forms cohesin between two sister chromatids at the centromeric heterochromatin, which then binds the centromeric heterochromatin, Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes, pombe |
| Immunogen: |
Amino acids 1-140 of human CENPA were used as the immunogen for the CENPA antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CENPA antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CENPA |
| Short name: |
CENPA Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CENPA (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1851 |
| Gene: |
CENPA |
More about : CENPA |
| Long gene name: |
centromere protein A |
| Synonyms gene name: |
17kDa , centromere protein A (17kD) centromere protein A |
| Synonyms: |
CENP-A CenH3 |
| Synonyms name: |
centromere-specific histone histone H3-like centromeric protein A |
| Locus: |
2p23, 3 |
| Discovery year: |
1988-05-31 |
| GenBank acession: |
U14518 |
| Entrez gene record: |
1058 |
| RefSeq identity: |
NM_001809 |
| Havana BLAST/BLAT: |
OTTHUMG00000097073 |