| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
VEGFR1 |
| Specificity: |
This antibody reacts specifically with VEGFR1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Cytoplasm |
| Antigen Source: |
KLH conjugated synthetic peptide derived from rat VEGFR1 |
| Gene ID: |
54251 |
| Swiss Prot: |
P53767 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1, Can promote endothelial cell proliferation, Has very high affinity for VEGFA and relatively low protein kinase activity, Its function in promoting cell proliferation seems to be cell-type specific, Ligand binding leads to the activation of several signaling cascades, MAPK3/ERK1 and the MAP kinase signaling pathway, May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, Mediates activation of MAPK1/ERK2, Mediates phosphorylation of PIK3R1, Modulates KDR signaling by forming heterodimers with KDR, Phosphorylates SRC, Promotes PGF-mediated proliferation of endothelial cells, Promotes phosphorylation of AKT1 and PTK2/FAK1 (By similarity), VEGFB and PGF, YES1 and PLCG, and cancer cell invasion, and may also phosphorylate CBL, and plays an essential role in the development of embryonic vasculature, and proliferation of some types of cancer cells, as well as of the AKT1 signaling pathway, but does not promote proliferation of normal fibroblasts, cell migration, cell survival, chemotaxis, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway, macrophage function, may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR, survival and angiogenesis in adulthood, the regulation of angiogenesis, the regulatory subunit of phosphatidylinositol 3-kinase, 4, 5-trisphosphate and the activation of protein kinase C, Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
VEGFR1 |
| Also known as: |
VEGFR1 Polyclonal Antibody |
| Other name: |
Anti-VEGFR1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
VEGFR1 |
| Short name: |
Anti-VEGFR1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-VEGFR1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
3763 |
| Gene: |
FLT1 |
More about : FLT1 |
| Long gene name: |
fms related tyrosine kinase 1 |
| Synonyms gene: |
FLT |
| Synonyms gene name: |
fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) fms-related tyrosine kinase 1 |
| Synonyms name: |
vascular endothelial growth factor receptor 1 vascular permeability factor receptor |
| Locus: |
13q12, 3 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
AF063657 |
| Entrez gene record: |
2321 |
| Pubmed identfication: |
2158038 |
| RefSeq identity: |
NM_002019 |
| Classification: |
I-set domain containing Immunoglobulin like domain containing Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000016648 |
| Locus Specific Databases: |
The Globin Gene Server LRG_425 |