| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Versican |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
1-0, 5-1ug/ml, 5ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Optimal dilution of the Versican antibody should be determined by the researcher |
| Intented use: |
This Versican antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P13611 |
| Purity: |
Antigen affinity |
| Description: |
Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, The Versican gene is mapped on 5q14, The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1, The human versican gene contains 15 exons spanning more than 90 kb, Versican is a large chondroitin sulfate proteoglycan, Versican staining was noted in the central and peripheral nervous system, also known as CSPG2, and on the luminal surface of some glandular epithelia, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14, in the basal layer of the epidermis, is a large extracellular matrix proteoglycan that is present in a variety of human tissues, versican strongly enhances LLC metastatic growth, which is upregulated in many human tumors including lung cancer, 2-q14, 3, Versican (VCAN) |
| Immunogen: |
Amino acids KWSKIEVDKNGKDLKETTVLVAQNGNIK of human Versican were used as the immunogen for the Versican antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Versican antibody may be kept for up to one month refrigerated at +4 degrees C, After reconstitution, For long-term |
| Localization: |
secreted, Intracellular |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Versican |
| Short name: |
Versican Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Versican (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2464 |
| Gene: |
VCAN |
More about : VCAN |
| Long gene name: |
versican |
| Synonyms gene: |
CSPG2 |
| Synonyms gene name: |
chondroitin sulfate proteoglycan 2 |
| Synonyms: |
PG-M |
| Synonyms name: |
versican proteoglycan |
| Locus: |
5q14, 2-q14, 3 |
| Discovery year: |
1991-07-18 |
| GenBank acession: |
X15998 |
| Entrez gene record: |
1462 |
| Pubmed identfication: |
1478664 21063030 |
| RefSeq identity: |
NM_004385 |
| Classification: |
Sushi domain containing Hyalectan proteoglycans V-set domain containing C-type lectin domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000131321 |