| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Versican |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Versican antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Versican antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P13611 |
| Purity: |
Antigen affinity |
| Description: |
Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan Versican, By activating TLR2: TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, It is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000kDa, The distribution of Versican has been seen by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent Versican splice variants V0 and V1, The human gene contains 15 exons spanning more than 90 kb, Versican staining was noted in the central and peripheral nervous system, Versican strongly enhances LLC metastatic growth, also known as CSPG2, and on the luminal surface of some glandular epithelia, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14, in the basal layer of the epidermis, is a large extracellular matrix proteoglycan that is present in a variety of human tissues, which is upregulated in many human tumors including lung cancer, Versican (VCAN) |
| Immunogen: |
mouse and rat), Amino acids 3314-3333 (RYEINSLIRYHCKDGFIQRH) were used as the immunogen for this Versican antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Versican antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Versican |
| Short name: |
Versican Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Versican (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2464 |
| Gene: |
VCAN |
More about : VCAN |
| Long gene name: |
versican |
| Synonyms gene: |
CSPG2 |
| Synonyms gene name: |
chondroitin sulfate proteoglycan 2 |
| Synonyms: |
PG-M |
| Synonyms name: |
versican proteoglycan |
| Locus: |
5q14, 2-q14, 3 |
| Discovery year: |
1991-07-18 |
| GenBank acession: |
X15998 |
| Entrez gene record: |
1462 |
| Pubmed identfication: |
1478664 21063030 |
| RefSeq identity: |
NM_004385 |
| Classification: |
Sushi domain containing Hyalectan proteoglycans V-set domain containing C-type lectin domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000131321 |