| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Bub1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Bub1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Bub1 antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
699 |
| Purity: |
Antigen affinity |
| Description: |
Bub1 possesses versatile and distinct functions during the cell cycle, It was first identified in genetic screens of Saccharomyces cerevisiae, Loss-of-function mutations or absence of Bub1 has been reported to result in aneuploidy, The mitotic checkpoint kinase is evolutionary conserved in organisms as diverse as Saccharomyces cerevisiae and humans, The protein is bound to kinetochores and plays a key role in the establishment of the mitotic spindle checkpoint and chromosome congression, also known as Mitotic checkpoint serine/threonine kinase, chromosomal instability (CIN) and premature senescence, is an enzyme that in humans is encoded by the BUB1 gene, mainly in the SAC and chromosome alignment during metaphase, Budding uninhibited by benzimidazoles 1 |
| Immunogen: |
Human partial recombinant protein (AA 731-1085) was used as the immunogen for this Bub1 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Bub1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Bub1 |
| Short name: |
Bub1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
budding uninhibited by benzimidazoles 1 homolog (yeast) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1150 |
| Gene: |
BUB1L |
More about : BUB1L |
| Long gene name: |
BUB1 mitotic checkpoint serine/threonine kinase |
| Synonyms gene name: |
budding uninhibited by benzimidazoles 1 (yeast homolog) budding uninhibited by benzimidazoles 1 homolog (yeast) |
| Synonyms: |
hBUB1 BUB1A BUB1 |
| Locus: |
2q13 |
| Discovery year: |
1997-08-18 |
| GenBank acession: |
AF046078 |
| Entrez gene record: |
699 |
| RefSeq identity: |
NM_004336 |
| Havana BLAST/BLAT: |
OTTHUMG00000153638 |