| Long name: |
Bub1 Primary Polyclonal Antibody |
| Also known as: |
Bub1 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
Bub1 |
| Specificity: |
This is a highly specific antibody against Bub1 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
980-1030/1085 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Bub1 |
| Gene ID number: |
699 |
| Swiss Prot: |
O43683 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1), BUB1B, CENPE and MAD2L1, Can phosphorylate BUB3, Can regulate chromosome segregation in a kinetochore-independent manner, Has a key role in the assembly of checkpoint proteins at the kinetochore, Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity, Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis, Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function, Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion, Required for the kinetochore localization of PLK1, The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20, This complex can also phosphorylate MAD1L1, being required for the subsequent localization of CENPF, Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Bub1 |
| Short name: |
Bub1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
budding uninhibited by benzimidazoles 1 homolog (yeast) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1150 |
| Gene: |
BUB1L |
More about : BUB1L |
| Long gene name: |
BUB1 mitotic checkpoint serine/threonine kinase |
| Synonyms gene name: |
budding uninhibited by benzimidazoles 1 (yeast homolog) budding uninhibited by benzimidazoles 1 homolog (yeast) |
| Synonyms: |
hBUB1 BUB1A BUB1 |
| Locus: |
2q13 |
| Discovery year: |
1997-08-18 |
| GenBank acession: |
AF046078 |
| Entrez gene record: |
699 |
| RefSeq identity: |
NM_004336 |
| Havana BLAST/BLAT: |
OTTHUMG00000153638 |