| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Nephrin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-F, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Frozen): 0, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the Nephrin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Nephrin antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
4868 |
| Purity: |
Antigen affinity |
| Description: |
A defect in NPHS1 is associated with congenital nephrotic syndrome of the Finnish type and causes massive amounts of protein to be leaked into the urine, Nephrin is also required for cardiovascular development, The gene is primarily expressed in renal tissues, The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine, a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes, orproteinuria, The NPHS1 gene encodes for Nephrin |
| Immunogen: |
Human partial recombinant protein (AA 23-383) was used as the immunogen for this Nephrin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Nephrin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Nephrin |
| Short name: |
Nephrin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Nephrin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
7908 |
| Gene: |
NPHS1 |
More about : NPHS1 |
| Long gene name: |
NPHS1, nephrin |
| Synonyms gene name: |
Finnish type (nephrin) , congenital, nephrosis 1 |
| Synonyms: |
CNF NPHN |
| Locus: |
19q13, 12 |
| Discovery year: |
1994-12-14 |
| Entrez gene record: |
4868 |
| Pubmed identfication: |
9915943 9660941 |
| Classification: |
Fibronectin type III domain containing C2-set domain containing V-set domain containing Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000180689 |
| Locus Specific Databases: |
LRG_693 |