| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
PRMT4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the PRMT4 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This PRMT4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q86X55 |
| Purity: |
Antigen affinity |
| Description: |
AIB) of coactivators, CARM1/PRMT4 is a regulator of cyclin E1 and DHFR mRNA expression, GRIP1, It is responsible for moving cells toward the inner cell mass in developing blastocysts, Its main function includes catalyzing the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine, PRMT4 is a secondary coactivator through its association with p160 family (SRC-1, This gene also plays an important role in androgen receptors and may play a role in prostate cancer progression, This gene is mapped to 19p13, also known as PRMT4, as well as many other mammals, is an enzyme encoded by the CARM1 gene found in human beings, 2, CARM1 (coactivator-associated arginine methyltransferase 1) |
| Immunogen: |
mouse and rat), An amino acid sequence from the middle region of human CARM1/PRMT4 (HLAPFTDEQLYMEQFTKA) was used as the immunogen for this PRMT4 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the PRMT4 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
PRMT4 |
| Short name: |
PRMT4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
PRMT4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
23393 |
| Gene: |
CARM1 |
More about : CARM1 |
| Long gene name: |
coactivator associated arginine methyltransferase 1 |
| Synonyms: |
PRMT4 |
| Locus: |
19p13, 2 |
| Discovery year: |
2003-11-14 |
| GenBank acession: |
AF055027 |
| Entrez gene record: |
10498 |
| Pubmed identfication: |
10381882 11724789 |
| RefSeq identity: |
NM_199141 |
| Classification: |
Protein arginine methyltransferases |
| Havana BLAST/BLAT: |
OTTHUMG00000180699 |