| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Nicastrin |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Nicastrin antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Nicastrin antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q92542 |
| Purity: |
Antigen affinity |
| Description: |
It is mapped to 1q23, NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex, Nicastrin has also been identified as a regulator of neprilysin, The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, The encoded protein cleaves integral membrane proteins, This gene is not catalytically active, all of which undergo significant post-translational modification before becoming active in the cell, also known as NCSTN, an enzyme involved in the degradation of amyloid beta fragment, and may be a stabilizing cofactor required for gamma-secretase complex assembly, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretaseprotein complex, including Notch receptors and beta-amyloid precursor protein, is aproteinthat is a part of the gamma secretase protein complex, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimers disease, 2, Nicastrin |
| Immunogen: |
An amino acid sequence from the N-terminus of human NCSTN (VIHVVEKEEDLQWVLTD) was used as the immunogen for this Nicastrin antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Nicastrin antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Nicastrin |
| Short name: |
Nicastrin Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Nicastrin (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
17091 |
| Gene: |
NCSTN |
More about : NCSTN |
| Long gene name: |
nicastrin |
| Synonyms: |
KIAA0253 APH2 |
| Locus: |
1q23, 2 |
| Discovery year: |
2005-02-08 |
| GenBank acession: |
AF240468 |
| Entrez gene record: |
23385 |
| Pubmed identfication: |
9039502 10993067 |
| RefSeq identity: |
NM_015331 |
| Havana BLAST/BLAT: |
OTTHUMG00000033110 |
| Locus Specific Databases: |
LRG_223 |