| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MBD1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus) , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the MBD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MBD1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UIS9 |
| Purity: |
Antigen affinity |
| Description: |
2, Chromatin immunoprecipitation analysis revealed that MBD1 linked MCAF to methylated promoters, Deletion analysis revealed that the C-terminal transcriptional repressor domain(TRD) of MBD1 interacted with a conserved C-terminal domain of MCAF, Fujita et al, Hendrich et al, Reporter gene assays showed that MCAF increased the repressive function of the isolated TRD of MBD1 against SP1, Sumoylation did not alter the intracellular localization of MBD1 at nuclear foci in C-33A human cervical cancer cells, Uchimura et al, Using PCR on a hybrid panel and FISH, Using yeast 2-hybrid analysis, also known as PCM1 or CXXC3, and protein pull-down assays, is a protein that in humans is encoded by the MBD1 gene, reciprocal immunoprecipitation analysis, (1999) mapped the MBD1 gene to chromosome 18q21, (2003) showed that MBD1 interacted directly with MCAF, (2006) found that MBD1 was multiply sumoylated in HeLa cells, 1 cM distal to MBD2, Methyl-CpG-Binding Domain Protein 1 |
| Immunogen: |
An amino acid sequence from the middle region of human Methyl-CpG-Binding Domain Protein 1 (NQKRQKCRWRQCLQFA) was used as the immunogen for this MBD1 antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MBD1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MBD1 |
| Short name: |
MBD1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MBD1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6916 |
| Gene: |
MBD1 |
More about : MBD1 |
| Long gene name: |
methyl-CpG binding domain protein 1 |
| Synonyms: |
PCM1 CXXC3 |
| Locus: |
18q21, 1 |
| Discovery year: |
1997-10-29 |
| GenBank acession: |
Y10746 |
| Entrez gene record: |
4152 |
| Pubmed identfication: |
9207790 10441743 |
| RefSeq identity: |
NM_015846 |
| Classification: |
Methyl-CpG binding domain containing Zinc fingers CXXC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000132669 |