| Long name: |
MBD1 Primary Polyclonal Antibody |
| Also known as: |
MBD1 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
MBD1 |
| Specificity: |
This is a highly specific antibody against MBD1 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human MBD1 |
| Gene ID number: |
4152 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract, MBD1, MBD2, MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal, MBD3, MBD3 does not bind methylated DNA both in vivo and in vitro, MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases, MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA, Surprisingly, The demethylation enzyme was recently found to be a mammalian protein, The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol, The methylation pattern involves the enzymatic processes of methylation and demethylation, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, is imperative to proper development and regulates gene expression, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, which are known to be deficient in MeCP1 activity, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD), DNA methylation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
CXXC3, MBD 1, MBD1, MECP1 COMPLEX, Methyl CpG binding domain protein 1, Methyl CpG binding domain protein 1 isoform PCM1, Methyl CpG binding protein MBD1, Methyl CpG binding protein splice variant 1, Methyl CpG binding protein splice variant 2, Methyl CpG binding protein splice variant 3, Methyl CpG binding protein splice variant 4, PCM 1, PCM1, Protein containing methyl CpG binding domain 1, RFT, Regulator of fibroblast growth factor 2 FGF 2 transcription, The regulator of fibroblast growth factor 2FGF 2 transcription, CXXC 3 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MBD1 |
| Short name: |
MBD1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MBD1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6916 |
| Gene: |
MBD1 |
More about : MBD1 |
| Long gene name: |
methyl-CpG binding domain protein 1 |
| Synonyms: |
PCM1 CXXC3 |
| Locus: |
18q21, 1 |
| Discovery year: |
1997-10-29 |
| GenBank acession: |
Y10746 |
| Entrez gene record: |
4152 |
| Pubmed identfication: |
9207790 10441743 |
| RefSeq identity: |
NM_015846 |
| Classification: |
Methyl-CpG binding domain containing Zinc fingers CXXC-type |
| Havana BLAST/BLAT: |
OTTHUMG00000132669 |