| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
beta Glucuronidase |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the beta Glucuronidase antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This beta Glucuronidase antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P08236 |
| Purity: |
Antigen affinity |
| Description: |
It is a tetrameric glycoprotein composed of identical subunits, Miller et al, T-Beta-glucuronic acid acts naturally to cleave glucuronides from conjugated bilirubin to produce unconjugated bilirubin, The GUSB gene is mutated in mucopolysaccharidosis type VII, Tomatsu et al, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans, approximately 90% of which were point mutations, which is then absorbed into the bloodstream, (1990) reported that the gene is 21 kb long and contains 12 exons, (2001) postulated that the beta-Glucuronidase inhibitor L-aspartic acid can thus block enterohepatic circulation of bilirubin, (2002) stated that more than 45 different mutations in the GUSB gene had been identified in human patients with MPS VII, Beta-Glucuronidase is an enzyme that in humans is encoded by the GUSB gene, Kreamer et al |
| Immunogen: |
An amino acid sequence from the N-terminus of human GUSB (DFSDNRRRGFEEQWYRRPLWE) was used as the immunogen for this beta Glucuronidase antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the beta Glucuronidase antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
beta Glucuronidase |
| Short name: |
beta Glucuronidase Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
b Glucuronidase (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
4696 |
| Gene: |
GUSB |
More about : GUSB |
| Long gene name: |
glucuronidase beta |
| Synonyms gene name: |
beta , glucuronidase |
| Locus: |
7q11, 21 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M15182 |
| Entrez gene record: |
2990 |
| Pubmed identfication: |
3468507 |
| RefSeq identity: |
NM_000181 |
| Havana BLAST/BLAT: |
OTTHUMG00000023735 |