| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ABCA1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the ABCA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ABCA1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95477 |
| Purity: |
Antigen affinity |
| Description: |
ABC proteins transport various molecules across extra- and intracellular membranes, Addition of potential lipid substrates or lipid acceptors did not modify the ATPase activity or nucleotide occlusion by ABCA1, Dot blot analysis of 50 tissues revealed ubiquitous expression of ABCA1 mRNA, Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes, Mg(2+)-dependent ATP binding and low basal ATPase activity, Szakacs et al, Szakacs et al, The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters, This protein is a member of the ABCA subfamily, Using human ABCA1 expressed in the membrane fraction of sf9 insect cells, With cholesterol as its substrate, adrenal glands, also known as ABC1, and all fetal tissues examined, and bone marrow, and lowest expression in kidney, and the Cholesterol efflux regulatory protein (CERP), found specific, is a protein which in humans is encoded by the ABCA1 gene, liver, lung, mammary gland, member 1, pancreas, pituitary, speculated that it may be a regulatory protein or may require other protein partners for full activation, sub-family A, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway, with highest expression in placenta, ATP-binding cassette |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human ABCA1 (NFAKDQSDDDHLKDLSLH) was used as the immunogen for this ABCA1 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ABCA1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ABCA1 |
| Short name: |
ABCA1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
member 1 (Antibody to), sub-family A (ABC1), adenosine triphosphate-binding cassette |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ABC-1 and ABC1 and CERP and HDLDT1 and TGD, ABCA1 and IDBG-79441 and ENSG00000165029 and 19, ATPase binding, Abca1 and IDBG-147642 and ENSMUSG00000015243 and 11303, BT, Cell surfaces, engulfment and biological process this GO :0007040 and lysosome organization and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007584 and response to nutrient and biological process this GO :0008203 and cholesterol metabolic process and biological process this GO :0008509 and anion transmembrane transporter activity and molecular function this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010745 and negative regulation of macrophage derived foam cell differentiation and biological process this GO :0010875 and positive regulation of cholesterol efflux and biological process this GO :0010887 and negative regulation of cholesterol storage and biological process this GO :0015485 and cholesterol binding and molecular function this GO :0015914 and phospholipid transport and biological process this GO :0016197 and endosomal transport and biological process this GO :0016887 and ATPase activity and molecular function this GO :0017127 and cholesterol transporter activity and molecular function this GO :0019905 and syntaxin binding and molecular function this GO :0030139 and endocytic vesicle and cellular component this GO :0030301 and cholesterol transport and biological process this GO :0030819 and positive regulation of cAMP biosynthetic process and biological process this GO :0031267 and small GTPase binding and molecular function this GO :0032367 and intracellular cholesterol transport and biological process this GO :0032489 and regulation of Cdc42 protein signal transduction and biological process this GO :0033344 and cholesterol efflux and biological process this GO :0033700 and phospholipid efflux and biological process this GO :0034185 and apolipoprotein binding and molecular function this GO :0034186 and apolipoprotein A-I binding and molecular function this GO :0034188 and apolipoprotein A-I receptor activity and molecular function this GO :0034364 and high-density lipoprotein particle and cellular component this GO :0034380 and high-density lipoprotein particle assembly and biological process this GO :0034616 and response to laminar fluid shear stress and biological process this GO :0038027 and apolipoprotein A-I-mediated signaling pathway and biological process this GO :0042157 and lipoprotein metabolic process and biological process this GO :0042158 and lipoprotein biosynthetic process and biological process this GO :0042493 and response to drug and biological process this GO :0042632 and cholesterol homeostasis and biological process this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0043691 and reverse cholesterol transport and biological process this GO :0044255 and cellular lipid metabolic process and biological process this GO :0044281 and small molecule metabolic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045332 and phospholipid translocation and biological process this GO :0045335 and pha this GO cytic vesicle and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050702 and interleukin-1 beta secretion and biological process this GO :0051117 and ATPase binding and molecular function this GO :0055091 and phospholipid homeostasis and biological process this GO :0055098 and response to low-density lipoprotein particle stimulus and biological process this GO :0060155 and platelet dense granule organization and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071300 and cellular response to retinoic acid and biological process this GO :0071397 and cellular response to cholesterol and biological process, member 1, sub-family A (ABC1), this GO :0002790 and peptide secretion and biological process this GO :0005102 and receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005543 and phospholipid binding and molecular function this GO :0005548 and phospholipid transporter activity and molecular function this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006200 and ATP catabolic process and biological process this GO :0006497 and protein lipidation and biological process this GO :0006911 and pha this GO cytosis, this GO :0005102 : receptor binding, this GO :0005102 : receptor binding and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0005543 : phospholipid binding and also this GO :0005548 : phospholipid transporter activity and also this GO :0008509 : anion transmembrane transporter activity and also this GO :0015485 : cholesterol binding and also this GO :0016887 : ATPase activity and also this GO :0017127 : cholesterol transporter activity and also this GO :0019905 : syntaxin binding and also this GO :0031267 : small GTPase binding and also this GO :0034185 : apolipoprotein binding and also this GO :0034186 : apolipoprotein A-I binding and also this GO :0034188 : apolipoprotein A-I receptor activity and also this GO :0051117 : ATPase binding, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0005543 : phospholipid binding, this GO :0005548 : phospholipid transporter activity, this GO :0008509 : anion transmembrane transporter activity, this GO :0015485 : cholesterol binding, this GO :0016887 : ATPase activity, this GO :0017127 : cholesterol transporter activity, this GO :0019905 : syntaxin binding, this GO :0031267 : small GTPase binding, this GO :0034185 : apolipoprotein binding, this GO :0034186 : apolipoprotein A-I binding, this GO :0034188 : apolipoprotein A-I receptor activity, this GO :0051117 : ATPase binding, 89249 and IDBG-637291 and ENSBTAG00000020661 and 535379, ATP-binding cassette |
| Identity: |
29 |
| Gene: |
ABCA1 |
More about : ABCA1 |
| Long gene name: |
ATP binding cassette subfamily A member 1 |
| Synonyms gene: |
ABC1 HDLDT1 |
| Synonyms gene name: |
ATP-binding cassette, member 1 , sub-family A (ABC1) |
| Synonyms: |
TGD |
| Synonyms name: |
Tangier disease |
| Locus: |
9q31, 1 |
| Discovery year: |
2005-03-17 |
| GenBank acession: |
AJ012376 |
| Entrez gene record: |
19 |
| Pubmed identfication: |
8088782 10431236 10431237 10431238 |
| RefSeq identity: |
NM_005502 |
| Classification: |
ATP binding cassette subfamily A |
| Havana BLAST/BLAT: |
OTTHUMG00000020417 |