| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
14-3-3 sigma |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the 14-3-3 sigma antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This 14-3-3 sigma antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P31947 |
| Purity: |
Antigen affinity |
| Description: |
It was most abundant in tissues enriched in stratified keratinizing epithelium, Leffers et al, The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1, The protein called stratifin was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells, also known as 14-3-3 sigma, is strongly induced by gamma irradiation and other DNA-damaging agents, (1993) obtained a peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins, 8 kb upstream of its transcription start site, Stratifin (SFN) |
| Immunogen: |
mouse and rat), Amino acids 140-156 (KKRIIDSARSAYQEAMD) were used as the immunogen for this 14-3-3 sigma antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the 14-3-3 sigma antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
14-3-3 sigma |
| Short name: |
14-3-3 sigma Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
14-3-3 sigma (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12854 |
| Gene: |
YWHAQ |
More about : YWHAQ |
| Long gene name: |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| Synonyms gene name: |
theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein |
| Synonyms: |
HS1 14-3-3 |
| Synonyms name: |
protein tau |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-08-20 |
| GenBank acession: |
AF070556 |
| Entrez gene record: |
10971 |
| Pubmed identfication: |
11080204 |
| RefSeq identity: |
NM_006826 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000013848 |