| Category: |
Antibody |
| Concentration: |
05% sodium azide, 1 mg/ml BSA (US sourced) and 0, 5 mg/ml in 1X PBS with 0 |
| Form: |
Purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
14-3-3 sigma |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
Western blot: 1-3ug/ml |
| Notes: |
14-3-3 sigma antibody titration may be required for optimal results, Provided assay concentrations are suggestions only |
| Added buffer: |
pH 7, 1X Phosphate Buffered Saline (PBS), 4 |
| Intented use: |
This 14-3-3 sigma antibodyis to be used only for research purposes and not for diagnostics |
| Gene ID #: |
2810 |
| Purity: |
Purified by protein A Affinity Chromatography |
| Description: |
14-3-3 sigma, It is a tumor suppressor gene inactivated via DNA methylation, Reduced or no expression of 14-3-3 sigma is seen in various carcinoma including skin, There are seven 14-3-3 proteins in most mammals: beta, also called Stratifin, and have roles in a wide range of physiological processes including transcription, and transmembrane receptors, breast and prostate, cell cycle control and apoptosis, eta, gamma epsilon, including kinases, intracellular signaling, is a product of the SFN gene, phosphatases, sigma and tau, suggesting that 14-3-3 gene silencing may be involved in tumor progression, zeta, 14-3-3 proteins are regulators of a diverse group of signaling proteins |
| Immunogen: |
The amino acid sequence is common to the ~30kDa and ~20kDa isoforms, A recombinant protein fragment from the internal region of 14-3-3 sigma was used as the immunogen for this antibody |
| Storage: |
Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, Aliquot the 14-3-3 sigma antibody and store frozen at -20 deg |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
14-3-3 sigma |
| Short name: |
14-3-3 sigma Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
14-3-3 sigma (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
12854 |
| Gene: |
YWHAQ |
More about : YWHAQ |
| Long gene name: |
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta |
| Synonyms gene name: |
theta , theta polypeptide protein, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein |
| Synonyms: |
HS1 14-3-3 |
| Synonyms name: |
protein tau |
| Locus: |
2p25, 1 |
| Discovery year: |
1999-08-20 |
| GenBank acession: |
AF070556 |
| Entrez gene record: |
10971 |
| Pubmed identfication: |
11080204 |
| RefSeq identity: |
NM_006826 |
| Classification: |
14-3-3 phospho-serine/phospho-threonine binding proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000013848 |