| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ALK |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the ALK antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ALK antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
Q9UM73 |
| Purity: |
Antigen affinity |
| Description: |
ALK shows greatest sequence similarity to the insulin receptor subfamily of kinases, Expressed in the small intestine, Functional RNA interference screening on a set of these transcriptional targets revealed that CEBPB and BCL2A1 were absolutely necessary to induce cell transformation and/or to sustain growth and survival of ALK-positive ALCL cells, In Drosophila, It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system, Mutated ALK proteins were overexpressed, One particularly informative case presented a high-level gene amplification that was strictly limited to ALK, The deduced amino acid sequences reveal that ALK is a novel receptor tyrosine kinase having a putative transmembrane domain and an extracellular domain, The knockdown of expression in ALK-mutated cells, and brain but not in normal lymphoid cells, and showed constitutive kinase activity, but also in cell lines overexpressing the wildtype protein, hyperphosphorylated, indicating that this gene may contribute on its own to neuroblastoma development, is an enzyme that in humans is encoded by the ALK gene, led to a marked decrease of cell proliferation, localized Jeb activates Alk and the downstream Ras/mitogen-activated protein kinase cascade to specify a select group of visceral muscle precursors as muscle-patterning pioneers, testis, Anaplastic lymphoma kinase also known as CD246 (cluster of differentiation 246) |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human ALK (LFRLRHFPCGNVNYGYQQQ) was used as the immunogen for this ALK antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ALK antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ALK |
| Short name: |
ALK Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
anaplastic lymphoma receptor tyrosine kinase (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ALK and IDBG-42084 and ENSG00000171094 and 238, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, CD246 and NBLST3, Plasma membranes, this GO :0000187 and activation of MAPK activity and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004704 and NF-kappaB-inducing kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004714 and transmembrane receptor protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0007165 and signal transduction and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0007399 and nervous system development and biological process this GO :0008283 and cell proliferation and biological process this GO :0016020 and membrane and cellular component this GO :0016310 and phosphorylation and biological process this GO :0016772 and transferase activity, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004704 : NF-kappaB-inducing kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004714 : transmembrane receptor protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0016772 : transferase activity, this GO :0004704 : NF-kappaB-inducing kinase activity, this GO :0004713 : protein tyrosine kinase activity, this GO :0004714 : transmembrane receptor protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0016772 : transferase activity, transferase activity, transferring phosphorus-containing groups, transferring phosphorus-containing groups, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0038061 and NIK/NF-kappaB signaling and biological process this GO :0042981 and regulation of apoptotic process and biological process this GO :0043234 and protein complex and cellular component this GO :0046777 and protein autophosphorylation and biological process this GO :0048666 and neuron development and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, anaplastic lymphoma receptor tyrosine kinase |
| Identity: |
427 |
| Gene: |
ALK |
More about : ALK |
| Long gene name: |
anaplastic lymphoma receptor tyrosine kinase |
| Synonyms gene name: |
anaplastic lymphoma kinase (Ki-1) |
| Synonyms: |
CD246 |
| Locus: |
2p23, 1 , 2-p23 |
| Discovery year: |
1993-08-24 |
| GenBank acession: |
D45915 |
| Entrez gene record: |
238 |
| Pubmed identfication: |
8122112 |
| RefSeq identity: |
NM_004304 |
| Classification: |
CD molecules Receptor Tyrosine Kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000152034 |
| Locus Specific Databases: |
LRG_488 |