| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
SDHB |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the SDHB antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This SDHB antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P21912 |
| Purity: |
Antigen affinity |
| Description: |
In affected members of families with paragangliomas-4, It is one of four protein subunits forming succinate dehydrogenase, Pollard et al, SDHC and SDHD, Subunit B, The SDHB subunit is connected to the SDHA subunit on the hydrophilic, The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al(1995), and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, catalytic end of the SDH complex, iron sulfur protein is a protein that in humans is encoded by the SDHB gene, mutations were identified in the SDHB gene, respectively, the other three being SDHA, (2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, Succinate Dehydrogenase Complex |
| Immunogen: |
Amino acids 42-59 (FAIYRWDPDKAGDKPHMQ-human) were used as the immunogen for this SDHB antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the SDHB antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
SDHB |
| Short name: |
SDHB Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
SDHB (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
10681 |
| Gene: |
SDHB |
More about : SDHB |
| Long gene name: |
succinate dehydrogenase complex iron sulfur subunit B |
| Synonyms gene: |
SDH1 SDH |
| Synonyms gene name: |
iron sulfur (Ip) , iron sulfur (Ip) succinate dehydrogenase complex subunit B, subunit B, succinate dehydrogenase complex |
| Synonyms name: |
iron-sulfur subunit of complex II succinate dehydrogenase [ubiquinone] iron-sulfur subunit |
| Locus: |
1p36, 13 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
U17248 |
| Entrez gene record: |
6390 |
| RefSeq identity: |
NM_003000 |
| Classification: |
Mitochondrial complex II: succinate dehydrogenase subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000002289 |
| Locus Specific Databases: |
SDHB @ LOVD TCA Cycle Gene Mutation Database LRG_316 |