| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Integrin beta 4 ITGB4 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Integrin beta 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Integrin beta 4 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P16144 |
| Purity: |
Antigen affinity |
| Description: |
ITGB4 knockdown by short hairpin RNA countered ZKSCAN3-augmented anchorage-independent colony formation in the colon cancer cell lines, The ITGB4 gene is mapped on 17q25, The gene encodes the integrin beta 4 subunits, The integrin beta-4 subunit is characterized by an unusually long cytoplasmic domain that harbors 4 fibronectin type III (FNIII) repeats, They confirmed that ZKSCAN3 bound the promoter of ITGB4 in vitro and in vivo, This subunit tends to associate with alpha 6 subunits and is likely to play a pivotal role in the biology of invasive carcinoma, Using expression profiling, Vidal et al, Yang et al, a receptor for the laminins, also known as CD104 (Cluster of Differentiation 104), found compound heterozygosity for mutations in the gene in an infant with junctional epidermolysis bullosa associated with pyloric atresia, found that ITGB4 was upregulated 6-fold by ZKSCAN3 in transfected human colon cancer cells compared with parental cells, is a human gene, residing in 2 pairs separated by a connecting segment, 1, ITGB4 (Integrin beta 4) |
| Immunogen: |
An amino acid sequence from the C-terminus of human ITGB4 (TLSTHMDQQFFQT) was used as the immunogen for this Integrin beta 4 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Integrin beta 4 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Integrin beta 4 ITGB4 |
| Short name: |
Integrin beta 4 Antibody ITGB4 |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
b 4, Integrin b 4 (Antibody to) integrin |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CD104, Cell surfaces, ITGB4 and IDBG-643160 and ENSBTAG00000018169 and, ITGB4 and IDBG-68784 and ENSG00000132470 and 3691, Itgb4 and IDBG-214382 and ENSMUSG00000020758 and 192897, beta 4, protein binding, this GO :0001664 and G-protein coupled receptor binding and molecular function this GO :0004872 and receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005604 and basement membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006914 and autophagy and biological process this GO :0007154 and cell communication and biological process this GO :0007155 and cell adhesion and biological process this GO :0007160 and cell-matrix adhesion and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007275 and multicellular organismal development and biological process this GO :0008305 and integrin complex and cellular component this GO :0009611 and response to wounding and biological process this GO :0009925 and basal plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0030056 and hemidesmosome and cellular component this GO :0030198 and extracellular matrix organization and biological process this GO :0031252 and cell leading edge and cellular component this GO :0031581 and hemidesmosome assembly and biological process this GO :0034329 and cell junction assembly and biological process this GO :0043235 and receptor complex and cellular component this GO :0046847 and filopodium assembly and biological process this GO :0048870 and cell motility and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0001664 : G-protein coupled receptor binding, this GO :0001664 : G-protein coupled receptor binding and also this GO :0004872 : receptor activity and also this GO :0005515 : protein binding, this GO :0004872 : receptor activity, this GO :0005515 : protein binding, integrin |
| Identity: |
6158 |
| Gene: |
ITGB4 |
More about : ITGB4 |
| Long gene name: |
integrin subunit beta 4 |
| Synonyms gene name: |
beta 4 , integrin |
| Synonyms: |
CD104 |
| Locus: |
17q25, 1 |
| Discovery year: |
1991-08-06 |
| Entrez gene record: |
3691 |
| Pubmed identfication: |
2070796 |
| Classification: |
Integrin beta subunits CD molecules Fibronectin type III domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000179814 |