| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
XRCC1 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
ICC, IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Immunocytochemistry: 0, Western blot: 0 |
| Notes: |
Titration of the XRCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This XRCC1 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P18887 |
| Purity: |
Antigen affinity |
| Description: |
A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity, It may play a role in DNA processing during meiogenesis and recombination in germ cells, The XRCC1 gene is mapped to 19q13, The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents, The protein interacts with DNA ligase III, XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of ser518, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain, complementing defective, in chinese hamster 1 is a DNA repair protein which complexes with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway, thr519, 31, X-ray repair |
| Immunogen: |
An amino acid sequence from the N-terminus of human XRCC1 (QDSTHCAENLLKADTYRKWR) was used as the immunogen for this XRCC1 antibody (100% mouse homology) |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the XRCC1 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
XRCC1 |
| Short name: |
XRCC1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
X-ray repair complementing defective repair in Chinese hamster cells 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
RCC, XRCC1 and IDBG-55268 and ENSG00000073050 and 7515, XRCC1 and IDBG-639023 and ENSBTAG00000016268 and 616905, Xrcc1 and IDBG-155000 and ENSMUSG00000051768 and 22594, nuclei, protein binding, this GO :0000012 and single strand break repair and biological process this GO :0001666 and response to hypoxia and biological process this GO :0003684 and damaged DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005654 and nucleoplasm and cellular component this GO :0006281 and DNA repair and biological process this GO :0006284 and base-excision repair and biological process this GO :0006974 and cellular response to DNA damage stimulus and biological process this GO :0010033 and response to organic substance and biological process this GO :0021766 and hippocampus development and biological process this GO :0042493 and response to drug and biological process, this GO :0003684 : damaged DNA binding, this GO :0003684 : damaged DNA binding and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, X-ray repair complementing defective repair in Chinese hamster cells 1 |
| Identity: |
12828 |
| Gene: |
XRCC1 |
More about : XRCC1 |
| Long gene name: |
X-ray repair cross complementing 1 |
| Synonyms gene: |
RCC |
| Synonyms gene name: |
X-ray repair complementing defective repair in Chinese hamster cells 1 |
| Locus: |
19q13, 2 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
M36089 |
| Entrez gene record: |
7515 |
| Pubmed identfication: |
2247054 |
| RefSeq identity: |
NM_006297 |
| Havana BLAST/BLAT: |
OTTHUMG00000182699 |
| Locus Specific Databases: |
LRG_784 |