| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Mineralocorticoid Receptor |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Mineralocorticoid Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Mineralocorticoid Receptor antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P08235 |
| Purity: |
Antigen affinity |
| Description: |
11beta-hydroxysteroid dehydrogenase type 2(11beta-HSD2), Activation of the mineralocorticoid receptor, In epithelial tissues, In intact animals, It also responds to some progestins, It belongs to the nuclearreceptor family where the ligand diffuses into cells, MR is expressed in many tissues, Na+/K+ pump, Spironolactone and eplerenone are MR antagonists, The protein is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes, and an excretion of potassium to maintain a normal salt concentration in the body, and as a consequence an increase in extracellular volume, brown adipose tissue and sweat glands, central nervous system(hippocampus), colon, group C, heart, homodimerization and binding to hormone response elements present in the promoter of some genes, increase in blood pressure, interacts with the MR and results in a signal transduction affecting specific gene expression in the nucleus, its activation leads to the expression of proteins regulating ionic and water transports(mainly the epithelial sodium channel or ENaC, like cortisol, member 2) also known as MR (mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31, results in its translocation to the cell nucleus, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, such as the kidney, that converts cortisol to inactive cortisone, the MR is protected from glucocorticoids by co-localization of an enzyme, upon the binding of its ligand aldosterone, 1-31, 2, NR3C2 (nuclear recepttor subfamily 3 |
| Immunogen: |
mouse and rat), Amino acids 966-984 (DQLPKVESGNAKPLYFHRK-human) were used as the immunogen for this Mineralocorticoid Receptor antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Mineralocorticoid Receptor antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Mineralocorticoid Receptor |
| Short name: |
Mineralocorticoid Receptor Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Mineralocorticoid Receptor (Antibody to) |
| Alternative technique: |
antibodies |