| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
CBS |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the CBS antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This CBS antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P35520 |
| Purity: |
Antigen affinity |
| Description: |
A mutation that deletes the C-terminal 145 amino acids of CBS could restore activity of several mutant alleles found in homocystinurics, The catalytic domain of the protein is located in the N-terminal 409 amino acids, The enzyme is a homotetramer of 63-kD subunits and requires pyridoxal phosphate and heme for activity, The gene, The human CBS protein can substitute for the endogenous yeast CBS protein in Saccharomyces cerevisiae, and a regulatory domain is located in the C-terminal 142 amino acids, contains 23 exons, ranging in size from 42 to 299 bp, which is mapped to chromosome 21q22, Cystathionine Beta-Synthase catalyzes the first irreversible step of transsulfuration |
| Immunogen: |
An amino acid sequence from the middle region of human CBS (KWFKSNDEEAFTFARMLIAQE) was used as the immunogen for this CBS antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the CBS antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
CBS |
| Short name: |
CBS Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
CBS (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
1550 |
| Gene: |
CBS |
More about : CBS |
| Long gene name: |
cystathionine-beta-synthase |
| Synonyms: |
HIP4 |
| Locus: |
21q22, 3 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
L14577 |
| Entrez gene record: |
875 |
| Pubmed identfication: |
9790750 |
| RefSeq identity: |
NM_000071 |
| Havana BLAST/BLAT: |
OTTHUMG00000086834 |
| Locus Specific Databases: |
Cystathionine beta-synthase database LRG_777 |