| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
ADAM10 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the ADAM10 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This ADAM10 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O14672 |
| Purity: |
Antigen affinity |
| Description: |
ADAM proteins contain an N-terminal signal sequence, ADAM10 is a member of the ADAM family and members of this family are cell surface proteins with a unique structure possessing both potential adhesion and protease function, ADAM10 possesses TNFA convertase activity, Conversion of the membrane-bound precursor to a secreted mature protein is mediated by a protease termed TNFA convertase, The gene is mapped to chromosome 15q21, a cysteine-rich region, a disintegrin-like domain, a metalloprotease-like domain, a transmembrane domain, an EGF -like repeat, and a C-terminal cytoplasmic tail, followed by a prodomain, 3-q23, A Disintegrin and Metalloproteinase Domain 10 is also know as AD10 |
| Immunogen: |
An amino acid sequence from the C-terminus of human ADAM10 (SVQWSRHFSGRTITLQ) was used as the immunogen for this ADAM10 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the ADAM10 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
ADAM10 |
| Short name: |
ADAM10 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
ADAM metallopeptidase domain 10 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AD10 and AD18 and CD156c and HsT18717 and kuz and MADM and RAK, ADAM10 and IDBG-14155 and ENSG00000137845 and 102, ADAM10 and IDBG-646594 and ENSBTAG00000005481 and 282132, Adam10 and IDBG-182594 and ENSMUSG00000054693 and 11487, nuclei, protein homodimerization activity, this GO :0001701 and in utero embryonic development and biological process this GO :0004175 and endopeptidase activity and molecular function this GO :0004222 and metalloendopeptidase activity and molecular function this GO :0005102 and receptor binding and molecular function this GO :0005178 and integrin binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005798 and this GO lgi-associated vesicle and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006468 and protein phosphorylation and biological process this GO :0006508 and proteolysis and biological process this GO :0006509 and membrane protein ectodomain proteolysis and biological process this GO :0007162 and negative regulation of cell adhesion and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007219 and Notch signaling pathway and biological process this GO :0007220 and Notch receptor processing and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008219 and cell death and biological process this GO :0008237 and metallopeptidase activity and molecular function this GO :0008270 and zinc ion binding and molecular function this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0010820 and positive regulation of T cell chemotaxis and biological process this GO :0014069 and postsynaptic density and cellular component this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0017124 and SH3 domain binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0022617 and extracellular matrix disassembly and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030307 and positive regulation of cell growth and biological process this GO :0030335 and positive regulation of cell migration and biological process this GO :0030574 and collagen catabolic process and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0042117 and monocyte activation and biological process this GO :0042803 and protein homodimerization activity and molecular function this GO :0045087 and innate immune response and biological process this GO :0051088 and PMA-inducible membrane protein ectodomain proteolysis and biological process this GO :0051089 and constitutive protein ectodomain proteolysis and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0097038 and perinuclear endoplasmic reticulum and cellular component this GO :0097197 and tetraspanin-enriched microdomain and cellular component, this GO :0004175 : endopeptidase activity, this GO :0004175 : endopeptidase activity and also this GO :0004222 : metalloendopeptidase activity and also this GO :0005102 : receptor binding and also this GO :0005178 : integrin binding and also this GO :0005515 : protein binding and also this GO :0008237 : metallopeptidase activity and also this GO :0008270 : zinc ion binding and also this GO :0017124 : SH3 domain binding and also this GO :0019901 : protein kinase binding and also this GO :0042803 : protein homodimerization activity, this GO :0004222 : metalloendopeptidase activity, this GO :0005102 : receptor binding, this GO :0005178 : integrin binding, this GO :0005515 : protein binding, this GO :0008237 : metallopeptidase activity, this GO :0008270 : zinc ion binding, this GO :0017124 : SH3 domain binding, this GO :0019901 : protein kinase binding, this GO :0042803 : protein homodimerization activity, ADAM metallopeptidase domain 10 |
| Identity: |
188 |
| Gene: |
ADAM10 |
More about : ADAM10 |
| Long gene name: |
ADAM metallopeptidase domain 10 |
| Synonyms gene name: |
a disintegrin and metalloproteinase domain 10 |
| Synonyms: |
kuz MADM HsT18717 CD156c |
| Locus: |
15q21, 3 |
| Discovery year: |
1997-03-21 |
| GenBank acession: |
AF009615 |
| Entrez gene record: |
102 |
| Pubmed identfication: |
9344679 9441766 |
| RefSeq identity: |
NM_001110 |
| Classification: |
ADAM metallopeptidase domain containing CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000132633 |