| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
MEK3 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
5-1ug/ml, 5-1ug/ml, IHC (Paraffin): 0, Western blot: 0 |
| Notes: |
Titration of the MEK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This MEK3 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P46734 |
| Purity: |
Antigen affinity |
| Description: |
Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, It phosphorylates and thus activates MAPK14/p38-MAPK, Rampoldi et al, The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family, This kinase can be activated by insulin, This kinase is activated by mitogenic and environmental stress, and confers oncogenic transformation of primary cells, and is necessary for the expression of glucose transporter, and participates in the MAP kinase-mediated signaling cascade, which thus leads to the constitutive activation of MAPK14, (1997) localized the MAP2K3 gene to 17q11, 2, Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene |
| Immunogen: |
mouse and rat), An amino acid sequence from the C-terminus of human MEK3 (MEHPFFTLHKTKKTD) was used as the immunogen for this MEK3 antibody (100% homologous in human |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the MEK3 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
MEK3 |
| Short name: |
MEK3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
MEK3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6843 |
| Gene: |
MAP2K3 |
More about : MAP2K3 |
| Long gene name: |
mitogen-activated protein kinase kinase 3 |
| Synonyms gene: |
PRKMK3 |
| Synonyms: |
MEK3 MKK3 MAPKK3 |
| Synonyms name: |
MAPK/ERK kinase 3 MAP kinase kinase 3 dual specificity mitogen activated protein kinase kinase 3 |
| Locus: |
17p11, 2 |
| Discovery year: |
1997-11-11 |
| GenBank acession: |
L36719 |
| Entrez gene record: |
5606 |
| Pubmed identfication: |
9465908 |
| RefSeq identity: |
NM_145109 |
| Classification: |
Mitogen-activated protein kinase kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000134322 |