| Antigen detectable with this ELISA kit: |
Amphiregulin (AREG) |
| Species reactivity: |
Mus musculus (Mouse) |
| Range of detection: |
125pg/mL, 15, 250pg/mL, 31, 500pg/mL, 15, 62, 25pg/mL, 5pg/mL, 625-1000pg/mL The standard curve concentrations used for the ELISA&rsquo, 625pg/mL, s were 1000pg/mL |
| Sensitivity: |
5pg/mL, The minimum detectable dose of this kit is typically less than 5 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
cell culture supernates and other biological fluids, cell lysates, plasma, tissue homogenates, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amphiregulin (AREG), The concentration of Amphiregulin (AREG) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amphiregulin (AREG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain Amphiregulin (AREG), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
Amphiregulin (AREG) |
| Short name: |
Amphiregulin (AREG) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
Amphiregulin (amphiregulin) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
AR and AREGB and CRDGF and SDGF, AREG and IDBG-24392 and ENSG00000109321 and 374, Areg and IDBG-179096 and ENSMUSG00000029378 and 11839, BT, growth factor activity, nuclei, this GO :0005125 and cytokine activity and molecular function this GO :0005154 and epidermal growth factor receptor binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005615 and extracellular space and cellular component this GO :0005622 and intracellular and cellular component this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007186 and G-protein coupled receptor signaling pathway and biological process this GO :0007267 and cell-cell signaling and biological process this GO :0008083 and growth factor activity and molecular function this GO :0008283 and cell proliferation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0009986 and cell surface and cellular component this GO :0014009 and glial cell proliferation and biological process this GO :0014070 and response to organic cyclic compound and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0031175 and neuron projection development and biological process this GO :0032355 and response to estradiol and biological process this GO :0042327 and positive regulation of phosphorylation and biological process this GO :0042542 and response to hydrogen peroxide and biological process this GO :0043434 and response to peptide hormone and biological process this GO :0045668 and negative regulation of osteoblast differentiation and biological process this GO :0045740 and positive regulation of DNA replication and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051591 and response to cAMP and biological process this GO :0060598 and dichotomous subdivision of terminal units involved in mammary gland duct morphogenesis and biological process this GO :0060744 and mammary gland branching involved in thelarche and biological process this GO :0060749 and mammary gland alveolus development and biological process this GO :0060750 and epithelial cell proliferation involved in mammary gland duct elongation and biological process, this GO :0005125 : cytokine activity, this GO :0005125 : cytokine activity and also this GO :0005154 : epidermal growth factor receptor binding and also this GO :0005515 : protein binding and also this GO :0008083 : growth factor activity, this GO :0005154 : epidermal growth factor receptor binding, this GO :0005515 : protein binding, this GO :0008083 : growth factor activity, 28934 and IDBG-643038 and ENSBTAG00000018134 and 538751, amphiregulin |
| Identity: |
651 |
| Gene: |
AREG |
More about : AREG |
| Long gene name: |
amphiregulin |
| Synonyms gene: |
SDGF AREGB |
| Synonyms gene name: |
schwannoma-derived growth factor amphiregulin B |
| Locus: |
4q13, 3 |
| Discovery year: |
1989-05-19 |
| GenBank acession: |
M30704 |
| Entrez gene record: |
374 |
| Classification: |
Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000130006 |