| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Topoisomerase II alpha |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Topoisomerase II alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Topoisomerase II alpha antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
P11388 |
| Purity: |
Antigen affinity |
| Description: |
Addition of purified topoisomerase II-alpha reconstituted chromatin-dependent activation activity in reactions with core pol II, DNA topoisomerase II-alpha is associated with the pol II holoenzyme and is a required component of chromatin-dependent coactivation, DNA topoisomerases are enzymes that control and alter the topologic states of DNA in both prokaryotes and eukaryotes, Furthermore, It appears likely that the reaction catalyzed by topoisomerase II involves the crossing-over of 2 DNA segments, Specific inhibitors of topoisomerase II blocked transcription on chromatin templates, The TOP2A gene spans approximately 30 kb and contains 35 exons, There are about 100, Topoisomerase II from eukaryotic cells catalyzes the relaxation of supercoiled DNA molecules, and unknotting of circular DNA, but did not affect transcription on naked templates, catenation, constituting about 0, decatenation, knotting, 000 molecules of topoisomerase II per HeLa cell nucleus, 1% of the nuclear extract, The human topoisomerase II enzyme is encoded by a single-copy gene which is mapped to 17q21-q22 |
| Immunogen: |
An amino acid sequence from the C-terminus of human Topoisomerase II alpha (RKPSTSDDSDSNFEK) was used as the immunogen for this Topoisomerase II alpha antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Topoisomerase II alpha antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Topoisomerase II alpha |
| Short name: |
Topoisomerase II alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Topoisomerase II a (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11989 |
| Gene: |
TOP2A |
More about : TOP2A |
| Long gene name: |
topoisomerase (DNA) II alpha |
| Synonyms gene: |
TOP2 |
| Synonyms gene name: |
topoisomerase (DNA) II alpha 170kDa |
| Locus: |
17q21, 2 |
| Discovery year: |
1989-03-23 |
| Entrez gene record: |
7153 |
| Classification: |
Topoisomerases |
| Havana BLAST/BLAT: |
OTTHUMG00000155008 |