| Category: |
Antibody |
| Concentration: |
2ml sterile DI water, 5mg/ml if reconstituted with 0 |
| Form: |
Antigen affinity purified |
| Conjugation: |
Unconjugated |
| Clone: |
Polyclonal antibody |
| Recognised antigen: |
Mitofusin 2 |
| Host animal: |
Rabbit (Oryctolagus cuniculus) |
| Clonality: |
Polyclonal (rabbit origin) |
| Species reactivity: |
Due to limited knowledge and inability to test the antibody against all known species, Mouse (Mus musculus), Rat , we cannot guarantee that no other cross reactivity can occur, Human (Homo sapiens) |
| Tested applications: |
WB |
| Recommended dilutions: |
5-1ug/ml, Western blot: 0 |
| Notes: |
Titration of the Mitofusin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity, The stated application concentrations are suggested starting amounts |
| Intented use: |
This Mitofusin 2 antibodyis to be used only for research purposes and not for diagnostics |
| Uniprot #: |
O95140 |
| Purity: |
Antigen affinity |
| Description: |
Axonal CMT type 2A and autosomal dominant HMSN VI are caused by MFN2 and mutations in MFN2, It is expressed in many tissues and cell lines such as brain and KG-1 with the highest expression in heart and skeletal muscle, It is mapped to chromosome 1 and encodes a 757-amino acid protein that contains an ATP/GTP-binding site motif, It shares 60% identity with MFN1, MFN2 colocalizes with mitochondrial markers, This protein contains an N-terminal GTPase domain and a transmembrane domain near the C terminus, When stably expressed in COS-7 cells, which emphasizes its important role of mitochondrial function for both optic atrophies and peripheral neuropathies, Mitofusin 2(MFN2) is a mitochondrial transmembrane GTPase regulating mitochondrial fusion and that the nucleotide-dependent activation of MFN2 concomitantly protects the organelle from permeability transition |
| Immunogen: |
An amino acid sequence from the N-terminus of human Mitofusin 2 (SRCNSIVTVKKNKRHMAEV) was used as the immunogen for this Mitofusin 2 antibody |
| Storage: |
Avoid repeated freezing and thawing, Celcius or lower, Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity, For long-term, Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized, aliquot and store at -20 deg, the Mitofusin 2 antibody can be stored for up to one month at 4oC, After reconstitution |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24° |
| Gene target: |
Mitofusin 2 |
| Short name: |
Mitofusin 2 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
Mitofusin 2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16877 |
| Gene: |
MFN2 |
More about : MFN2 |
| Long gene name: |
mitofusin 2 |
| Synonyms: |
CPRP1 KIAA0214 MARF CMT2A2 |
| Locus: |
1p36, 22 |
| Discovery year: |
2003-02-25 |
| GenBank acession: |
AF036536 |
| Entrez gene record: |
9927 |
| Pubmed identfication: |
12499352 11181170 |
| RefSeq identity: |
NM_014874 |
| Havana BLAST/BLAT: |
OTTHUMG00000002392 |
| Locus Specific Databases: |
Inherited Peripheral Neuropathies Mutation Database LRG_255 |