| Antigen detectable with this ELISA kit: |
25-Hydroxyvitamin D3 (HVD3) |
| Species reactivity: |
General |
| Range of detection: |
2, 2, 7, 22, 66, 22ng/mL, 41ng/mL, 47-200ng/mL The standard curve concentrations used for the ELISA&rsquo, 47ng/mL, 67ng/mL, s were 200ng/mL |
| Sensitivity: |
88ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma and other biological fluids, Serum |
| Time for performing t hourse test: |
2, 5 hoursours |
| Test principle: |
A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled 25-Hydroxyvitamin D3 (HVD3) and unlabeled 25-Hydroxyvitamin D3 (HVD3) (Standards or samples) with the pre-coated antibody specific for 25-Hydroxyvitamin D3 (HVD3), After addition of the substrate solution, After incubation the unbound conjugate is washed off, An antibody specific for 25-Hydroxyvitamin D3 (HVD3) has been pre-coated onto a microplate, The amount of bound HRP conjugate is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample, the intensity of color developed is reverse proportional to the concentration of 25-Hydroxyvitamin D3 (HVD3) in the sample, This assay employs the competitive inhibition enzyme immunoassay technique |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
25-Hydroxyvitamin D3 (HVD3) |
| Short name: |
25-Hydroxyvitamin D3 (HVD3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
25-Hydroxyvitamin D3 (HVD3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |