| Antigen detectable with this ELISA kit: |
25-hydroxy vitamin D3 (25 HVD3) |
| Species reactivity: |
Rattus norvegicus (Rat) |
| Range of detection: |
20 ug/L-100 ug/L |
| Sensitivity: |
5 ug/L |
| Application: |
Ready to use kit for performing Enzyme-Linked Immunosorbent Assay (ELISA) |
| Sample types tested for this ELISA kit: |
serum |
| Time for performing t hourse test: |
1-5 hours |
| Test principle: |
", A set of standards is used to build a standard curve from which the amount of the antigen present in the samples and controls can be directly obtained, After a second washing step, The absorbance is measured on a microplate reader, The antigen from the sample and the standards are allowed to bind to the plate, The enzymatic reaction is terminated by addition of the stopping solution, The intensity of the color formed by the enzymatic reaction is directly proportional to the concentration of te antigen in the sample, This assay utilises two monoclonal antibodies of high specificity: A monoclonal antibody against the antigen of interest is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of the antigen is conjugated to horse radish peroxidase (HRP), a, and subsequently incubated with the HRP conjugate, the enzyme substrate is added, washed, The principle of this ELISA kit follows the typical capture in two steps, a, k, sandwich ELISA" |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| Gene target: |
25-hydroxy vitamin D3 (25 HVD3) |
| Short name: |
25-hydroxy vitamin D3 (25 HVD3) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells) |
| Alternative name: |
25-hydroxy vitamin D3 (25 HVD3) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |