| Also known as: |
b2-Microglobulin |
| Other names: |
G, G, HLA G antigen, HLA class I histocompatibility antigen, HLA class I molecule, HLA-G histocompatibility antigen, HLA-G protein, MHC class I antigen G, N/A, OTTHUMP00000029218, OTTHUMP00000029220, OTTHUMP00000033527, OTTHUMP00000159507, alpha chain G, b2 microglobulin, class I, class I, major histocompatibility complex, b2 microglobulin |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
HLA-G, MHC-G, N/A, HLA-G |
| Gene name synonims: |
HLA-G, MHC-G, N/A, HLA-G |
| Other gene names: |
HLA-G, MHC-G, N/A, HLA-G |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
8L771 |
| Host organism: |
Mouse (Mus musculus) |
| Source organism: |
Human (Homo sapiens) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Does not crossreact with Chicken, It is absent on erythrocytes, Mouse or Rabbit, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, Species Crossreactivity: Crossreacts with Human, This particular antibody might cross react with speacies outside of the listed ones, Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops, b2M is a 12kD Ig like glycoprotein expressed on lymphocytes, bovine, canine, endothelial cells and epithelial cells, granulocytes, monocytes, platelets, thymocytes, The antibody B2M-01 reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin |
| Purification method: |
Affinity Purified by immunoaffinity chromatography |
| Form/Appearance: |
pH 7, 2, Supplied as a liquid in PBS |
| Storage and shipping: |
Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius, For long-term storage and to avoid repeated freezing and thawing, For maximum recovery of product, Further dilutions can be made in assay buffer, aliquot For optimal long term storage, centrifuge the original vial after thawing and prior to removing the cap, the antibody should be kept at -20 degrees Celsius, Store the antibody at +4 degrees Celsius for short term storage |
| Tested applications:: |
Flow Cytometry (FC/FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
Beta 2 Microglobulin precursor, Beta Chain of MHC Class 1 Proteins, HDCMA22P), b2-Microglobulin (B2M |
| Short name: |
Beta 2 Microglobulin precursor, Beta Chain of MHC Class 1 Proteins, HDCMA22P) (FITC) Antibody, b2-Microglobulin (B2M |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
HDCMA22P) (fluorecein) (Antibody to), b 2 Microglobulin precursor, b epitope on Major histocompabitility complex Class 1 Proteins, b2-Microglobulin (beta-2-microglobulin |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
B2M and IDBG-646936 and ENSBTAG00000012330 and 280729, B2M and IDBG-9902 and ENSG00000166710 and 567, B2m and IDBG-202736 and ENSMUSG00000060802 and 12010, Extracellular, TAP-dependent and biological process this GO :0002480 and antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent and biological process this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0012507 and ER to this GO lgi transport vesicle membrane and cellular component this GO :0016020 and membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030670 and pha this GO cytic vesicle membrane and cellular component this GO :0031901 and early endosome membrane and cellular component this GO :0031905 and early endosome lumen and cellular component this GO :0033077 and T cell differentiation in thymus and biological process this GO :0042026 and protein refolding and biological process this GO :0042493 and response to drug and biological process this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0042612 and MHC class I protein complex and cellular component this GO :0042802 and identical protein binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0046686 and response to cadmium ion and biological process this GO :0050690 and regulation of defense response to virus by virus and biological process this GO :0050776 and regulation of immune response and biological process this GO :0060333 and interferon-gamma-mediated signaling pathway and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, TAP-independent and biological process this GO :0002481 and antigen processing and presentation of exogenous protein antigen via MHC class Ib, identical protein binding, this GO :0000139 and this GO lgi membrane and cellular component this GO :0001895 and retina homeostasis and biological process this GO :0001916 and positive regulation of T cell mediated cytotoxicity and biological process this GO :0002237 and response to molecule of bacterial origin and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0042802 : identical protein binding, this GO :0042802 : identical protein binding, 783680, beta-2-microglobulin |
| Identity: |
914 |
| Gene: |
B2M |
More about : B2M |
| Long gene name: |
beta-2-microglobulin |
| Locus: |
15q21, 1 |
| Discovery year: |
2001-06-22 |
| GenBank acession: |
AB021288 |
| Entrez gene record: |
567 |
| RefSeq identity: |
NM_004048 |
| Classification: |
C1-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000131247 |