| Long name: |
62) Monoclonal Antibody, 68, Zap70 (1484CT290 |
| Also known as: |
62) Monoclonal Antibody, 68, Zap70 (1484CT290 |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
Zap70 |
| Specificity: |
This is a highly specific antibody against Zap70 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Monoclonal |
| Clone number: |
1484CT290, 62, 68 |
| Source: |
This Zap70 antibody is generated from a mouse immunized with a recombinant protein |
| Gene ID number: |
22637 |
| Swiss Prot: |
P43404 |
| Tested Applications: |
FCM, IF, WB |
| Recommended dilutions: |
FC(1:25), IF(1:25) , WB(1:2000) |
| Cross reactivity: |
Mouse, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
Additionally, Contributes also to the development and activation of primary B-lymphocytes, During thymocyte development, Furthermore, In turn, Regulates motility, Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK, Subsequently, T-cell proliferation and differentiation, This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation, Thus, When antigen presenting cells (APC) activate T-cell receptor (TCR), ZAP70 controls cytoskeleton modifications, ZAP70 is also required for TCR-CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative), ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR), a large number of signaling molecules are recruited and ultimately lead to lymphokine production, a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD3Z through ITAM motif at the plasma membrane, adhesion and cytokine expression of mature T-cells, adhesion and mobility of T-lymphocytes, as well as thymocyte development, thus ensuring correct delivery of effectors to the APC, Tyrosine kinase that plays an essential role in regulation of the adaptive immune response |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
70 kDa zeta-chain associated protein, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70, ZAP-70, Zap70, mrtle, mur, Srk |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Gene target: |
62) Monoclonal, 68, Zap70 (1484CT290 |
| Short name: |
62) Monoclonal Antibody, 68, Zap70 (1484CT290 |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
62) monoclonal (Antibody to), 68, zeta-chain (TCR) associated protein kinase 70kDa (1484CT290 |
| Alternative technique: |
antibodies |