| Also known as: |
MHC CLASS I MONOMORPHIC |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
CVS22 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Horse |
| Specificity and cross-reactivity: |
Clone CVS22 recognises monomorphic equine MHC Class I and was classified at the International Equine Leucocyte Antigen Workshop, In horses, MHC class I is expressed by all nucleated cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, this complex is referred to as the equine leukocyte antigen (ELA) region, MHC CLASS I MONOMORPHIC The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, Storage in frost-free freezers is not recommended, for longer, for one month or the antibody should be stored at -20 degrees Celsius, the antibody should be stored undiluted, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
MHC CLASS I MONOMORPHIC: |
| Short name: |
MOUSE Anti-HORSE MHC CLASS I MONOMORPHIC:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
MOUSE antibody to-HORSE Major histocompabitility complex CLASS I MONOMORPHIC:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |