| Also known as: |
CD16/CD32 |
| Other names: |
Fc gamma RIIB, Fc gamma receptor IIB, Fc receptor, Fc-gamma RII, Fc-gamma-RII, Fc-gamma-RIIB, FcRII, FcgammaRIIB, IgG, IgG Fc receptor II beta, Low affinity immunoglobulin gamma Fc region receptor II, Lymphocyte antigen 17, fc gamma receptor IIB, fc-gamma RII, fc-gamma-RIIB, fcRII, igG Fc receptor II beta, low affinity IIb, low affinity immunoglobulin gamma Fc region receptor II-b, lymphocyte antigen 17, low affinity immunoglobulin gamma Fc region receptor II-b isoform 2 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD16/CD32 |
| Other gene names: |
AI528646, CD32, F630109E10Rik, Fc-gamma RII, Fc-gamma-RIIB, FcRII, Fc[g]RII, FcgRII, Fcgr2, Fcgr2, Fcgr2a, Fcgr2b, Fcr-2, Fcr-3, Ly-17, Ly-17, Ly-17, Ly-m20, LyM-1, Lym-1, Fcgr2b |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2b |
| Clone: |
FCR4G8 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes, Fc gamma III (CD16) and Fc gamma II (CD32), In the mouse only the transmembrane form of CD16 is reported to exist, NK cells, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, and subpopulations of T lymphocytes, granulocytes, myeloid precursors, which is expressed on macrophages, CD16/CD32 This item recognises an epitope expressed by the murine low affinity Fc receptors |
| Form/Appearance: |
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid) |
| Concentration: |
1 miligram per 1 mililiter, IgG concentration 0 |
| Storage and shipping: |
If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use, Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided, Shelf Life: 18 months from date of dispatch, the antibody is photosensitive and should be protected from light, the antibody should be stored undiluted, Storage in frost-free freezers is not recommended, Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term |
| Tested applications:: |
Flow Cytometry (FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Gene target: |
CD16/CD32: |
| Short name: |
RAT ANTI MOUSE CD16/CD32:FITC Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, Antibody |
| Label: |
FITC |
| Alternative name: |
RAT antibody to MOUSE CD16/CD32:fluorecein (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3620 |
| Gene: |
FCGR3B |
More about : FCGR3B |
| Long gene name: |
Fc fragment of IgG receptor IIIb |
| Synonyms gene: |
FCGR3 FCG3 |
| Synonyms gene name: |
Fc fragment of IgG, low affinity IIIb, low affinity IIIb, receptor (CD16b) , receptor for (CD16) Fc fragment of IgG |
| Synonyms: |
CD16 CD16b |
| Synonyms name: |
Fc gamma receptor IIIb |
| Locus: |
1q23, 3 |
| Discovery year: |
1991-08-21 |
| GenBank acession: |
J04162 |
| Entrez gene record: |
2215 |
| Pubmed identfication: |
2139735 |
| RefSeq identity: |
NM_000570 |
| Classification: |
CD molecules Immunoglobulin like domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000074099 |