| Long name: |
FITC Conjugated, ALAS1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ALAS1 |
| Specificity: |
This is a highly specific antibody against ALAS1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ALAS1/ALAS-H |
| Gene ID number: |
211 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ALAS-E, ALAS-H is a housekeeping protein which mediates synthesis of early heme in the mitochondria of most cells, NF-E2 and EKLF, Since the ALAS gene maps to the X chromosome, Succinyl CoA associates with ALAS-E in protein conformation change and translocation of ALAS-E into the mitochondria and does not interact with ALAS-H, The ALAS-E 5'-flanking region contains binding sites for nuclear activators such as GATA-1, The erythroid-specific isoenzyme, an enzyme catalyzing the first step of the heme biosynthetic pathway in mammals, mutation of the gene leads to the pyridoxine-refractory X-linked sideroblastic anemia, regulates the first step of hematopoietic cell differentation and iron metabolism in the liver, 5-aminolevulinate synthase 1 (ALAS-H) and 2 (ALAS-E) are two isoforms of ALAS |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ALAS1 Conjugated |
| Short name: |
FITC Conjugated, ALAS1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ALAS1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
396 |
| Gene: |
ALAS1 |
More about : ALAS1 |
| Long gene name: |
5', -aminolevulinate synthase 1 |
| Synonyms gene: |
ALAS3 ALAS |
| Synonyms gene name: |
aminolevulinate, delta-, synthase 1 |
| Locus: |
3p21, 2 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
X56351 |
| Entrez gene record: |
211 |
| Havana BLAST/BLAT: |
OTTHUMG00000158108 |