| Long name: |
FITC Conjugated, 5 MethylCytosine Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
5 MethylCytosine |
| Specificity: |
This is a highly specific antibody against 5 MethylCytosine |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated 5 MethylCytosine |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Others |
| Crossreactive species details: |
5 MethylCytosine |
| Antigen background: |
DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), Several enzymes, The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), a potent epigenetics marker and regulator of gene expression, and carboxy- (5-caC) cytidine, formyl- (5-fC), including the Tet family of proteins have been implicated in cytidine demethylation, Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5 m C, 5 mC, 5 me C, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-mC, 5-Methyl Cytidine |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
5 MethylCytosine Conjugated |
| Short name: |
FITC Conjugated, 5 MethylCytosine Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, 5 MethylCytosine (Antibody to) |
| Alternative technique: |
antibodies |