| Long name: |
Biotin Conjugated, 5 MethylCytosine Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
5 MethylCytosine |
| Specificity: |
This is a highly specific antibody against 5 MethylCytosine |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated 5 MethylCytosine |
| Tested applications: |
IHC-P |
| Recommended dilutions: |
IHC-P(1:100-500) |
| Crossreactivity: |
Others |
| Crossreactive species details: |
5 MethylCytosine |
| Antigen background: |
DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), Several enzymes, The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), a potent epigenetics marker and regulator of gene expression, and carboxy- (5-caC) cytidine, formyl- (5-fC), including the Tet family of proteins have been implicated in cytidine demethylation, Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5 m C, 5 mC, 5 me C, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine, 5-mC, 5-Methyl Cytidine |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
5 MethylCytosine Conjugated |
| Short name: |
Conjugated, 5 MethylCytosine Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, 5 MethylCytosine (Antibody to) |
| Alternative technique: |
antibodies |