| Long name: |
Biotin Conjugated, MARCH3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
37681 |
| Specificity: |
This is a highly specific antibody against 37681 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human MARCH3 |
| Gene ID number: |
115123 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Involved in the pathway of protein modification, MARCH3 (membrane-associated ring finger (C3HC4) 3), MARCH3 functions as an E3 ubiquitin-protein ligase that accepts a ubiquitin residue from an E2 ubiquitin-conjugating enzyme and is thought to be involved in endosomal trafficking events, The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), also known as RNF173, is a 253 amino acid multi-pass membrane protein that localizes to cytoplasmic vesicles and early endosomes and contains one RING-CH-type zinc finger, the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s), Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
MGC48332 MARCH-III RNF173 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MARCH3 Conjugated |
| Short name: |
Conjugated, MARCH3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
E3 ubiquitin protein ligase (Antibody to), biotinilated coupled, membrane-associated ring finger (C3HC4) 3 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
E3 ubiquitin protein ligase, MARCH3 and IDBG-39523 and ENSG00000173926 and 115123, MARCH3 and IDBG-644898 and ENSBTAG00000006797 and 520348, March3 and IDBG-148017 and ENSMUSG00000032656 and 320253, Plasma membranes, metal ion binding, this GO :0005515 and protein binding and molecular function this GO :0005764 and lysosome and cellular component this GO :0005768 and endosome and cellular component this GO :0006897 and endocytosis and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016567 and protein ubiquitination and biological process this GO :0016874 and ligase activity and molecular function this GO :0030659 and cytoplasmic vesicle membrane and cellular component this GO :0031901 and early endosome membrane and cellular component this GO :0046872 and metal ion binding and molecular function, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0046872 : metal ion binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0046872 : metal ion binding, membrane-associated ring finger (C3HC4) 3 |
| Identity: |
28728 |
| Gene: |
MARCH3 |
More about : MARCH3 |
| Long gene name: |
membrane associated ring-CH-type finger 3 |
| Synonyms gene name: |
E3 ubiquitin protein ligase , membrane-associated ring finger (C3HC4) 3 membrane-associated ring finger (C3HC4) 3 |
| Locus: |
5q23, 2 |
| Discovery year: |
2005-01-26 |
| GenBank acession: |
AF055007 |
| Entrez gene record: |
115123 |
| Pubmed identfication: |
14722266 8619474 |
| RefSeq identity: |
NM_178450 |
| Classification: |
Membrane associated ring-CH-type fingers Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000128968 |