| Long name: |
FITC Conjugated, MLF1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MLF1 |
| Specificity: |
This is a highly specific antibody against MLF1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human MLF1/2/3 |
| Gene ID number: |
4291 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus, Interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels, Suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest, Involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MLF1 Conjugated |
| Short name: |
FITC Conjugated, MLF1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, myeloid leukemia factor 1 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
DNA-templated and biological process this GO :0007050 and cell cycle arrest and biological process this GO :0019904 and protein domain specific binding and molecular function, MLF1 and IDBG-63091 and ENSG00000178053 and 4291, MLF1 and IDBG-637188 and ENSBTAG00000004126 and 533379, Mlf1 and IDBG-150128 and ENSMUSG00000048416 and 17349, nuclei, protein domain specific binding, this GO :0002318 and myeloid progenitor cell differentiation and biological process this GO :0003677 and DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0006351 and transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0005515 : protein binding and also this GO :0019904 : protein domain specific binding, this GO :0005515 : protein binding, this GO :0019904 : protein domain specific binding, myeloid leukemia factor 1 |
| Identity: |
7125 |
| Gene: |
MLF1 |
More about : MLF1 |
| Long gene name: |
myeloid leukemia factor 1 |
| Synonyms name: |
myeloid leukemia factor 1 variant 1 myeloid leukemia factor 1 variant 2 myeloid leukemia factor 1 variant 3 |
| Locus: |
3q25, 32 |
| Discovery year: |
1994-08-29 |
| GenBank acession: |
L49054 |
| Entrez gene record: |
4291 |
| Pubmed identfication: |
8570204 |
| RefSeq identity: |
NM_022443 |
| Havana BLAST/BLAT: |
OTTHUMG00000158775 |