| Long name: |
FITC Conjugated, Histone H2A, X (Ubiquityl Lys119) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Histone H2A, X Ubiquityl Lys119 |
| Specificity: |
This modification site is homologous to that of Lys119 in Rat, This is a highly specific antibody against Histone H2A, X Ubiquityl Lys119 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
90-130/143 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human Ubiquityl Histone H2A, X (Lys119) |
| Gene ID number: |
3014 |
| Swiss Prot: |
P16104 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
DNA accessibility is regulated via a complex set of post-translational modifications of histones, DNA repair, DNA replication and chromosomal stability, Histones thereby play a central role in transcription regulation, Nucleosomes wrap and compact DNA into chromatin, Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation, also called histone code, and nucleosome remodeling, limiting DNA accessibility to the cellular machineries which require DNA as a template, Variant histone H2A which replaces conventional H2A in a subset of nucleosomes |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
H2A, H2A/X, H2AFX, Histone H2A, Histone H2AX, H2AX, X, X |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Histone H2A, X (Ubiquityl Lys119) Conjugated |
| Short name: |
FITC Conjugated, Histone H2A, X (Ubiquityl Lys119) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Histone H2A, X (Ubiquityl Lys119) (Antibody to) |
| Alternative technique: |
antibodies |