| Long name: |
FITC Conjugated, UBE2J2 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
UBE2J2 |
| Specificity: |
This is a highly specific antibody against UBE2J2 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human UBE2J2 |
| Gene ID number: |
118424 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Localized to the membrane of the endoplasmic reticulum (ER), This ATP-dependent reaction yields AMP, Two isoforms of UBE2J2 exist due to alternative splicing events, UBE2J2 (Ubiquitin-conjugating enzyme E2 J2), UBE2J2 catalyzes the attachment of ubiquitin to misfolded membrane proteins, a diphosphate and a ubiquitin-tagged protein and may be a method of quality control within the ER, also known as NCUBE2 (Non-canonical ubiquitin-conjugating enzyme 2), is a 259 amino acid single pass type IV membrane protein that that belongs to the E2 ubiquitin-conjugating enzyme family and is involved in protein degradation, thereby targeting them for proteasomal destruction, ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s), Ubiquitination is an important molecular mechanism by which abnormal or short-lived proteins are targeted for degradation by the concerted efforts of at least three classes of enzymes: ubiquitin-activating enzymes (E1s) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Ubc6p NCUBE2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
UBE2J2 Conjugated |
| Short name: |
FITC Conjugated, UBE2J2 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, UBE2J2 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
19268 |
| Gene: |
UBE2J2 |
More about : UBE2J2 |
| Long gene name: |
ubiquitin conjugating enzyme E2 J2 |
| Synonyms gene name: |
J2 , J2 (UBC6 homolog, ubiquitin-conjugating enzyme E2, yeast) ubiquitin-conjugating enzyme E2 |
| Locus: |
1p36, 33 |
| Discovery year: |
2002-11-26 |
| GenBank acession: |
AF296658 |
| Entrez gene record: |
118424 |
| Pubmed identfication: |
11278356 |
| RefSeq identity: |
NM_058167 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000001911 |