| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
UBE2J2 |
| Specificity: |
This antibody reacts specifically with UBE2J2 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human UBE2J2 |
| Gene ID: |
118424 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Localized to the membrane of the endoplasmic reticulum (ER), This ATP-dependent reaction yields AMP, Two isoforms of UBE2J2 exist due to alternative splicing events, UBE2J2 (Ubiquitin-conjugating enzyme E2 J2), UBE2J2 catalyzes the attachment of ubiquitin to misfolded membrane proteins, a diphosphate and a ubiquitin-tagged protein and may be a method of quality control within the ER, also known as NCUBE2 (Non-canonical ubiquitin-conjugating enzyme 2), is a 259 amino acid single pass type IV membrane protein that that belongs to the E2 ubiquitin-conjugating enzyme family and is involved in protein degradation, thereby targeting them for proteasomal destruction, ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s), Ubiquitination is an important molecular mechanism by which abnormal or short-lived proteins are targeted for degradation by the concerted efforts of at least three classes of enzymes: ubiquitin-activating enzymes (E1s) |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
Ubc6p NCUBE2 |
| Also known as: |
UBE2J2 Polyclonal Antibody |
| Other name: |
Anti-UBE2J2 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
UBE2J2 |
| Short name: |
Anti-UBE2J2 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-UBE2J2 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
19268 |
| Gene: |
UBE2J2 |
More about : UBE2J2 |
| Long gene name: |
ubiquitin conjugating enzyme E2 J2 |
| Synonyms gene name: |
J2 , J2 (UBC6 homolog, ubiquitin-conjugating enzyme E2, yeast) ubiquitin-conjugating enzyme E2 |
| Locus: |
1p36, 33 |
| Discovery year: |
2002-11-26 |
| GenBank acession: |
AF296658 |
| Entrez gene record: |
118424 |
| Pubmed identfication: |
11278356 |
| RefSeq identity: |
NM_058167 |
| Classification: |
Ubiquitin conjugating enzymes E2 |
| Havana BLAST/BLAT: |
OTTHUMG00000001911 |