| Long name: |
FITC Conjugated, HSJ1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HSJ1 |
| Specificity: |
This is a highly specific antibody against HSJ1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human HSJ1/DNAJB2 |
| Gene ID number: |
3300 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein, DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, DnaJB2 (DnaJ homolog subfamily B member 2), The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis, The presence of the J domain defines a protein as a member of the DnaJ family, The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function, Two isoforms are produced due to alternative splicing, also known as HSJ1 or HSPF3, exocytosis and endocytosis, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus, The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
HSPF3 CMT2T |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HSJ1 Conjugated |
| Short name: |
FITC Conjugated, HSJ1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HSJ1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5228 |
| Gene: |
DNAJB2 |
More about : DNAJB2 |
| Long gene name: |
DnaJ heat shock protein family (Hsp40) member B2 |
| Synonyms gene: |
HSJ1 |
| Synonyms gene name: |
DnaJ (Hsp40) homolog, member 2 , subfamily B |
| Locus: |
2q35 |
| Discovery year: |
1997-08-15 |
| Entrez gene record: |
3300 |
| Pubmed identfication: |
1599432 10516435 |
| Classification: |
DNAJ (HSP40) heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000133134 |