| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
HSJ1 |
| Specificity: |
This antibody reacts specifically with HSJ1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human HSJ1/DNAJB2 |
| Gene ID: |
3300 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein, DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, DnaJB2 (DnaJ homolog subfamily B member 2), The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis, The presence of the J domain defines a protein as a member of the DnaJ family, The proteins contain cysteine rich regions that are composed of zinc fingers that form a peptide binding domain responsible for the chaperone function, Two isoforms are produced due to alternative splicing, also known as HSJ1 or HSPF3, exocytosis and endocytosis, is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus, The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
HSPF3 CMT2T |
| Also known as: |
HSJ1 Polyclonal Antibody |
| Other name: |
Anti-HSJ1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
HSJ1 |
| Short name: |
Anti-HSJ1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-HSJ1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
5228 |
| Gene: |
DNAJB2 |
More about : DNAJB2 |
| Long gene name: |
DnaJ heat shock protein family (Hsp40) member B2 |
| Synonyms gene: |
HSJ1 |
| Synonyms gene name: |
DnaJ (Hsp40) homolog, member 2 , subfamily B |
| Locus: |
2q35 |
| Discovery year: |
1997-08-15 |
| Entrez gene record: |
3300 |
| Pubmed identfication: |
1599432 10516435 |
| Classification: |
DNAJ (HSP40) heat shock proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000133134 |