| Long name: |
Biotin Conjugated, ID4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ID4 |
| Specificity: |
This is a highly specific antibody against ID4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ID4 |
| Gene ID number: |
3400 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Due to the absence of the basic region, Id2 (4), Id3 and Id4 (5), It has been shown that expression of each of the Id proteins is strongly dependent on growth factor activation and that reduction of Id mRNA levels by antisense oligonucleotides leads to a delayed reentry of arrested cells into the cell cycle following growth factor stimulation, Since Id1 inhibits DNA binding of E12 and Myo D, The Id-type proteins thus appear to negatively regulate DNA binding of bHLH proteins, They are ubiquitously expressed and dimerize with members of the class A and B HLH proteins (1â, Under conditions that facilitate muscle cell differentiation, allowing E12 and/or E47 to form heterodimers with Myo D and myogenin, it apparently functions to inhibit muscle-specific gene expression, the Id protein levels fall, the resulting heterodimers cannot bind DNA, which in turn activate myogenic differentiation, &euro, &euro, &ldquo, &ldquo, 3), 5), Members of the Id family of basic helix-loop-helix (bHLH) proteins include Id1 (1â |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
bHLHb27 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ID4 Conjugated |
| Short name: |
Conjugated, ID4 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, ID4 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
5363 |
| Gene: |
ID4 |
More about : ID4 |
| Long gene name: |
HLH protein , inhibitor of DNA binding 4 |
| Synonyms gene name: |
dominant negative helix-loop-helix protein , inhibitor of DNA binding 4 |
| Synonyms name: |
inhibitor of differentiation 4 |
| Locus: |
6p22, 3 |
| Discovery year: |
1995-02-13 |
| GenBank acession: |
U16153 |
| Entrez gene record: |
3400 |
| Pubmed identfication: |
7665172 |
| RefSeq identity: |
NM_001546 |
| Classification: |
Basic helix-loop-helix proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000014333 |