| Long name: |
ALEXA FLUOR 594 Conjugated, p70 S6 kinase alpha Polyclonal Antibody |
| Also known as: |
Anti-p70 S6 kinase alpha PAb ALEXA FLUOR 594 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
p70 S6 kinase alpha |
| Specificity: |
This is a highly specific antibody against p70 S6 kinase alpha |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
280-330/525 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human p70 S6 kinase alpha |
| Gene ID Number: |
6198 |
| Swiss Prot: |
P23443 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Also controls translation initiation by phosphorylating a negative regulator of EIF4A, Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, In cells lacking functional TSC1-2 complex, In response to IGF1, Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function, PDCD4, Phosphorylates mitochondrial URI1 leading to dissociation of a URI1-PPP1CC complex, Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR, RPS6 and EEF2K, Regulates protein synthesis through phosphorylation of EIF4B, The active form then phosphorylates and activates several substrates in the pre-initiation complex, The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, Under conditions of nutrient depletion, Upon mitogenic stimulation, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), and contributes to cell survival by repressing the pro-apoptotic function of BAD, cell growth and cell cycle progression, constitutively phosphorylates and inhibits GSK3B, including the EIF2B complex and the cap-binding complex component EIF4B, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation, resulting in accelerated degradation of IRS1, resulting in the inhibition of mTORC2 and AKT1 signaling, targeting it for ubiquitination and subsequent proteolysis, the inactive form associates with the EIF3 translation initiation complex, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function, which leads to its inhibition and thus activation of EEF2, Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
70 kDa ribosomal protein S6 kinase 1, P70S6K1, PS6K, RPS6KB1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, STK14A, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha, p70-S6K, p70-S6K 1, p70-alpha, S6K |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
594, ALEXA FLUOR®, Alexa Fluor |
| Conjugated: |
Alexa conjugate 1 |
| Gene target: |
p70 S6 kinase alpha |
| Short name: |
p70 S6 kinase alpha Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 594 |
| Alternative name: |
ALEXA FLUOR 594, p70 S6 phosphorylation catalyst a (Antibody to) |
| Alternative technique: |
antibodies |