| Long name: |
FITC Conjugated, BRF1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BRF1 |
| Specificity: |
This is a highly specific antibody against BRF1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human BRF1/RNA polymerase IIIB 90 kDa subunit |
| Gene ID number: |
2972 |
| Swiss Prot: |
Q92994 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
7SL and 5S RNA, Isoform 2 is required for transcription of the U6 promoter, The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
TFIIIB90 BRF hBRF |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
BRF1 Conjugated |
| Short name: |
FITC Conjugated, BRF1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, BRF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11551 |
| Gene: |
BRF1 |
More about : BRF1 |
| Long gene name: |
BRF1, RNA polymerase III transcription initiation factor subunit |
| Synonyms gene: |
TAF3B2 TAF3C GTF3B |
| Synonyms gene name: |
GTF3B subunit 2 BRF1 homolog, RNA polymerase III, RNA polymerase III transcription initiation factor 90 kDa subunit , TATA box binding protein (TBP)-associated factor, cerevisiae) BRF1, subunit of RNA polymerase III transcription initiation factor IIIB (S |
| Locus: |
14q32, 33 |
| Discovery year: |
1998-08-20 |
| GenBank acession: |
U28838 |
| Entrez gene record: |
2972 |
| Pubmed identfication: |
7624363 8943358 |
| RefSeq identity: |
NM_001519 |
| Classification: |
General transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000029884 |