| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
BRF1 |
| Specificity: |
This antibody reacts specifically with BRF1 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Subcellular locations: |
Nucleus |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human BRF1/RNA polymerase IIIB 90 kDa subunit |
| Gene ID: |
2972 |
| Swiss Prot: |
Q92994 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
7SL and 5S RNA, Isoform 2 is required for transcription of the U6 promoter, The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
TFIIIB90 BRF hBRF |
| Also known as: |
BRF1 Polyclonal Antibody |
| Other name: |
Anti-BRF1 Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
BRF1 |
| Short name: |
Anti-BRF1 (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-BRF1 (polyclonal) |
| Alternative technique: |
polyclonals |
| Identity: |
11551 |
| Gene: |
BRF1 |
More about : BRF1 |
| Long gene name: |
BRF1, RNA polymerase III transcription initiation factor subunit |
| Synonyms gene: |
TAF3B2 TAF3C GTF3B |
| Synonyms gene name: |
GTF3B subunit 2 BRF1 homolog, RNA polymerase III, RNA polymerase III transcription initiation factor 90 kDa subunit , TATA box binding protein (TBP)-associated factor, cerevisiae) BRF1, subunit of RNA polymerase III transcription initiation factor IIIB (S |
| Locus: |
14q32, 33 |
| Discovery year: |
1998-08-20 |
| GenBank acession: |
U28838 |
| Entrez gene record: |
2972 |
| Pubmed identfication: |
7624363 8943358 |
| RefSeq identity: |
NM_001519 |
| Classification: |
General transcription factors |
| Havana BLAST/BLAT: |
OTTHUMG00000029884 |