| Long name: |
FITC Conjugated, Bid (Ser61) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Bid Ser61 |
| Specificity: |
This is a highly specific antibody against Bid Ser61 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser61 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse Bid around the phosphorylation site of Ser61 |
| Gene ID number: |
12122 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas, Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, In animal model studies, Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway, Mutation in the SH3 domain can diminish the cytochrome c releasing activity, The cytochrome c releasing activity of Bid was antagonized by Bcl2, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor, which in turn induces cytochrome c release and mitochondrial damage, Bid |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
AltName: BH3-interacting domain death agonist p15, BH3 interacting domain death agonist, BH3-interacting domain death agonist, BH3-interacting domain death agonist p11, BH3-interacting domain death agonist p13, BID, p-Bid phospho S61, p11 BID, p13 BID, p22 BID, Bid phospho S61 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Bid (Ser61) Conjugated |
| Short name: |
FITC Conjugated, Bid (Ser61) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, BH3 interacting domain death a this GO nist (Ser61) (Antibody to) |
| Alternative technique: |
antibodies |