| Long name: |
FITC Conjugated, AEV polyprotein Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
AEV polyprotein |
| Specificity: |
This is a highly specific antibody against AEV polyprotein |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
2040-2100/2134 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from Av AEV polyprotein |
| Gene ID number: |
944582 |
| Swiss Prot: |
Q9YLS4 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Virus |
| Crossreactive species details: |
Avian Encephalomyelitis virus |
| Antigen background: |
AEV VP4 may not be myristoylated (By similarity), All these proteins contain a beta-sheet structure called beta-barrel jelly roll, In addition to its proteolytic activity, It acts as a genome-linked replication primer (By similarity), It displays RNA-binding, Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein, Protein 2B and 2BC precursor affect membrane integrity and cause an increase in membrane permeability, Protein 2C: Associates with and induces structural rearrangements of intracellular membranes, Protein 3A, Protein 3B is covalently linked to the 5'-end of both the positive-strand and negative-strand genomic RNAs, Protein VP0: VP0 precursor is a component of immature procapsids, RNA and substrate bind cooperatively to the protease (By similarity), RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals, The N-terminal domain of VP0, Together they form an icosahedral capsid (T=3) composed of 60 copies of each VP1, Unlike other picornaviruses, VP1 is situated at the 12 fivefold axes, VP2, VP2, and VP3, and VP3 form a closed capsid enclosing the viral positive strand RNA genome, and thus influences viral genome replication, is needed for the assembly of 12 pentamers into the icosahedral structure, it binds to viral RNA, nucleotide binding and NTPase activities (By similarity), protein VP4, serves as membrane anchor, via its hydrophobic domain, whereas VP2 and VP3 are located at the quasi-sixfold axes (By similarity), with a diameter of approximately 300 Angstroms, Capsid proteins VP1 |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Genome polyprotein |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
AEV polyprotein Conjugated |
| Short name: |
FITC Conjugated, AEV polyprotein Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, AEV polyprotein (Antibody to) |
| Alternative technique: |
antibodies |