| Long name: |
FITC Conjugated, Bub1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Bub1 |
| Specificity: |
This is a highly specific antibody against Bub1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
980-1030/1085 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human Bub1 |
| Gene ID number: |
699 |
| Swiss Prot: |
O43683 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1), BUB1B, CENPE and MAD2L1, Can phosphorylate BUB3, Can regulate chromosome segregation in a kinetochore-independent manner, Has a key role in the assembly of checkpoint proteins at the kinetochore, Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity, Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis, Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function, Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion, Required for the kinetochore localization of PLK1, The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20, This complex can also phosphorylate MAD1L1, being required for the subsequent localization of CENPF, Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Bub1 Conjugated |
| Short name: |
FITC Conjugated, Bub1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, budding uninhibited by benzimidazoles 1 homolog (yeast) (Antibody to) |
| Alternative technique: |
antibodies |